川银花-黄芩药对配伍前后指纹图谱的变化及其多指标成分的定量分析

Change of Fingerprints and Quantitative Analysis of Multi-index Constituents in Couplet Medicines of Lonicerae Similis Flos-Scutellariae Radix Before and After Compatibility

目的:研究川银花-黄芩药对配伍前后指纹图谱及指标性成分含量的变化,对该药对的临床应用有一定参考意义。方法:采用Inert Sustain C18色谱柱(4.6 mm×250 mm,5μm),流动相乙腈-0.2%磷酸溶液梯度洗脱,流速1.0 m L·min^(-1),柱温25℃,进样量10μL,检测波长分别为327 nm(0~27,43~62 min),237 nm(27~33 min),274 nm(33~43,90~120 min)和280 nm(62~90 min);分别建立10批川银花、黄芩单煎液及合煎液的指纹图谱,标定共有峰,获得各自对照指纹图谱。对配伍后指纹图谱的共有峰进行归属分析并通过11种混合对照品测定指标性成分的含量。结果:川银花-黄芩药对的指纹图谱标定26个共有峰,9个来源于川银花,17个来源于黄芩;色谱峰1,2,3,4,13,14,15,18,23,25,26分别对应新绿原酸,绿原酸,隐绿原酸,咖啡酸,异绿原酸B,异绿原酸A,异绿原酸C,黄芩苷,汉黄芩苷,黄芩素,汉黄芩素;配伍前后峰的数目未发生变化,但指标性成分的含量发生了改变。结论:川银花-黄芩药对配伍前后化学成分种类无差异,但主要有效成分的溶出会相互影响。

Objective： To assess difference of fingerprints and index constituents in couplet medicines of Lonicerae Similis Flos-Scutellariae Radix before and after compatibility. Method： The separation was performed on Inert Sustain C18column（ 4. 6 mm × 250 mm,5 μm） with mobile phase composed of acetonitrile-0. 2% phosphoric acid solution for gradient elution at the flow rate of 1. 0 m L·min-1,the column temperature was set at 25 ℃,the injection volume was 10 μL,the detection wavelengths were set at 327 nm（ 0-27,43-62 min）,237 nm（ 27-33 min）,274 nm（ 33-43,90-120 min）,280 nm（ 62-90 min）. The fingerprints of single and mixed decoction of 10 batches of Lonicerae Similis Flos and Scutellariae Radix were established,common peaks were demarcated and their respective control fingerprints were gained. Attributing analysis of common peaks in the fingerprints after compatibility was adopted and the contents of index ingredients through the 11 kinds of mixed control were determined. Result： Twenty-six common peaks were marked in the HPLC fingerprints of couplet medicines; 9derived from Lonicerae Similis Flos and 17 derived from Scutellariae Radix; the peaks of 1,2,3,4,13,14,15,18,23,25,26 corresponded to neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid,isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C, baicalin, wogonoside, baicalein,wogonin,respectively. The number of peaks had no change before and after compatibility,but the contents of index ingredients made differences. Conclusion： There are no differences in chemical ingredients from couplet medicines of Lonicerae Similis Flos-Scutellariae Radix before and after compatibility,but the dissolution of main effective components affects each other.