沉默MMP-10基因对人肺腺癌裸鼠皮下移植瘤的影响

Influence of MMP-10 gene silencing on subcutaneous tumor of human lung adenocarcinoma cells in nude mice

目的:探讨沉默基质金属蛋白酶-10(MMP-10)对人肺腺癌裸鼠皮下移植瘤的影响。方法:采用携带干扰序列的si RNA慢病毒沉默A549细胞MMP-10的表达,构建沉默MMP-10表达的稳转细胞株及裸鼠移植瘤模型,比较实验组(A549-si MMP-10组)和对照组(A549-NC组)裸鼠成瘤瘤体生长速度、瘤体体积及瘤重的差异。结果:A549-si MMP-10组裸鼠成瘤瘤体生长速度明显慢于A549-NC组,瘤体体积及瘤重明显小于A549-NC组,差异均有统计学意义(均P<0.05)。结论:MMP-10基因可能与人肺腺癌的发生、发展密切相关,MMP-10基因有望成为判断人肺腺癌预后的一个新的重要指标。

Objective： To investigate the effect of matrix metalloproteinases-10 （MMP-10） gene silencing on sub- cutaneous tumor of human lung adenocarcinoma cells in nude mice. Methods： Adopting lentivirus of siRNA which carry interference sequence to silence the expression of MMP-10 gene in A549 cells, building MMP-10 si- lence stable cells strain and nude-mouse transplanted tumor model, and comparing the differences of growth speed, size, and weight of nude mouse with subcutaneous tumor between the experimental group （A549-si MMP-10） and the control group （A549-NC）. Results： The growth speed of tumor from A549-si MMP-10 group was significantly slower than the A549-NC group, as well as the size and weight （P〈0. 05）. Conclusion： MMP-10 genes might relate to the occurrence and development of adenocarcinoma cells of human lung.