急性移植物抗宿主病患者血浆miR-155的表达及其临床意义

Expression and clinical significance of plasma miR-155 in the patients with acute graftversus-host disease

目的:探讨急性移植物抗宿主病(aGVHD)患者miR-155的表达水平及其临床意义。方法:采用造血干细胞移植患者移植第0、第7、第14、第21及第28天的血浆标本,实时荧光定量PCR(qPCR)方法检测miR-155相对表达量,酶联免疫吸附试验(ELISA)法检测患者血浆中白细胞介素(IL)-4、IL-7、IL-10的含量。结果:miR-155在移植后第7天开始逐渐升高,到第28天时升高明显,患者移植后不同时间点miR-155相对表达量比较差异具有统计学意义(P<0.05)。allo-HSCT后不同时间点IL-4、IL-7、IL-10水平比较,差异均无统计学意义(P>0.05)。结论:allo-HSCT后miR-155较IL-4、IL-7、IL-10水平变化出现早,可作为早期诊断和预测a GVHD的无创性生物标记物。

Objective： To evaluate the expression and clinical significance of miR-155 in patients with acute graft-versus-host disease （aGVHD）. Methods： miRNA was extracted from the plasma samples of patients day 0, day 7, day 14, day 21, day 28 after their hematopoietic stem cell transplantation（HSCT）. The expressions of miR- 155, interleukin （IL） -4, IL-7 and IL- 10 were detected by qPCR. Results： The expression of miR- 155 obvi- ously increased on day 7 after HSCT, and reached the peak on day 28. The miR-155 expression level was in- creased with the prolonged time after transplantation （P〈0.05）. There was no significant difference in the ex- pression of IL-4, IL-7 and IL-10 between any time points（P〉0. 05）. Conclusion： MiR-155 might be a nonin- vasion biomarker for early detection of aGVHD, and the level of miR-155 changed earlier than IL-4, IL-10 and IL-7 after HSCT.