麻鸭感染Ⅰ群禽腺病毒4型的致病及防治研究

Prevention and treatment of the disease in Shelduck resulted from a fowl adenovirus type 4 infection

2016年6月,山东省某鸭场养殖的麻鸭在25日龄时发生一种以猝死、心包积液和肝坏死为特征的疾病,死亡率高达40%。通过临床表现,结合PCR检测、鸭腺病毒排查检测、鸡胚传代分离培养、血凝性试验、SPF鸡回归试验与PCR血清型定位分析等实验室检测,确诊该病为Ⅰ群禽腺病毒4型(FAdV-4)感染所致。为有效防控该病,首先注射超免疫卵黄抗体进行治疗,短期内虽然起到了积极的治疗作用,但保护期后该病复发,死亡数量增加,转而采集发病鸭肝组织并进行研磨、灭活,制备组织灭活疫苗,实施紧急免疫,最终成功控制了该病。结果表明,该麻鸭场感染FAdV-4导致发生鸭的心包积水综合征,而同源组织灭活疫苗紧急免疫是防治该病的重要手段。

In June 2016,a highly lethal infectious disease emerged in a farm of 25-day-old shelduck locating in Shandong Province,which was characterized as sudden death,hydropericardium and liver necrosis,and the mortality rate reached up to 40%.The disease was diagnosed based on clinical signs,PCR detection,differential detection of duck adenovirus,isolation and cultivation in SPF chicken embryo passage,hemagglutination test,SPF chicken regression test and serotype mapping based on PCR sequences analysis.The results showed that the fowl adenovirus type 4（FAdV-4） was the agent for this disease.In order to prevent and control the disease,the chicken egg-yolk antibodies were injected firstly for treatment,which had a positive therapeutic effect in a short term.The disease recurred with the increasing death number,then the liver of dead ducks was collected and the tissue inactivated vaccine was developed,and all animal were performed contingency vaccination,and finally,the disease was successfully controlled.The ducks in this farm were infected with FAdV-4,which resulted in hydropericardium,and contingency vaccination of the homologous tissue inactivated vaccine is an important way for disease prevention and treatment.