基于基因芯片技术新疆汉族食管鳞癌差异表达mRNA的筛选

Screening of mRNA differentially expressed genes in esophageal squamous cell carcinoma of Xinjiang Han population based on cDNA microarray technology

目的食管癌是全球常见的的恶性肿瘤,新疆为发病率较高的地区之一。本研究旨在完善新疆汉族食管鳞癌(esophageal squamous cell carcinoma,ESCC)基因表达谱,进一步了解ESCC的发生、发展机制,提供与ESCC的预防、诊断及治疗相关的新的生物标志。方法收集2014-11-03-2015-09-25新疆石河子大学医学院第一附属医院兵团内镜中心6例汉族中分化ESCC患者的癌组织和癌旁正常组织(距癌组织>5cm)。利用基因芯片技术检测新疆汉族ESCC的差异表达基因。结果共筛选出汉族ESCC差异表达的mRNAs 335个,其中有138个表达上调的mRNAs和197个表达下调的mRNAs,差异倍数(fold change,FC)≥2且P<0.01,这些基因涉及多种生物学功能及通路。结论利用基因芯片技术基于人类全基因谱筛选了新疆汉族ESCC差异表达基因,完善了新疆汉族ESCC基因谱,为ESCC的诊断及预后的进一步研究打下基础。

OBJECTIVE Esophageal cancer is one of the most common malignant tumors in the world, and Xinjiang is one of the areas with higher incidence. This study aimed to improve the gene expression profile of esophageal squamous cell carcinoma in Xinjiang Han population, and further understand the mechanism of the occurrence and development of ESCC, and find new biological markers associated with the prevention, diagnosis and treatment of ESCC. METHODS cDNA microarray technology was used to analyze six pairs of ESCC cancer tissues and adjacent normal tissue in the Xinjiang Han patients, in order to detect differentially expressed genes and improve gene expression profile in ESCC in Xinjiang Han patients. RESULTS The study screened a total of 335 mRNAs which were differently expressed in Han patients with ESCC, with 138 over-expressed mRNAs and 197 down-expressed mRNAs（FC≥2, P〈0.01）. Simultane- ously, these genes involved a variety of biological functions and pathways. CONCLUSIONS Based on human whole ge- nome, we use gene cDNA microarray technology to screening of differentially expressed genes in esophageal squamous cell carcinoma in Xinjiang Han population, and improve gene expression profile of esophageal squamous cell carcinoma, lay the foundation for further research on the diagnosis and prognosis of esophageal squamous cell carcinoma.