纳米铜/石墨烯-壳聚糖复合膜修饰电极及其对葡萄糖的直接测定

Preparation of Nano-copper/Graphene-Chitosan Composite Membrane Modified Electrode and Its Direct Determination of Glucose

以石墨烯-壳聚糖复合膜修饰玻碳电极,并在此复合膜上电沉积纳米铜,用于葡萄糖的无酶检测。以扫描电镜、傅立叶红外光谱及电化学交流阻抗谱对该复合膜微观形态进行表征,以循环伏安法、计时电流法对该电极的电化学行为进行研究。实验结果表明,在0.1 mol/L Na OH溶液中修饰电极对葡萄糖具有良好的催化氧化作用,该电极对葡萄糖的检测线性范围为5.6×10-5~1.2×10-3mol/L,检出限(S/N=3)为2.3×10-5mol/L。该修饰电极对样品的检测具有良好的稳定性、重现性。

The enzyme-free detection of glucose was achieved by using the nano-copper/graphene-chitosan composite matrix modified glass carbon electrode.The microstructure of the composite matrix was characterized by scanning electron microscopy（SEM）,FT-IR spectrum and electrochemical impedance spectroscopy（EIS）.The electrochemistry behavior of the modified electrode was investigated by cyclic voltammetry and chronoamperometry.The results showed that the modified electrode exhibited a good catalytic oxidation of glucose in 0.1 mol/L NaOH and with a wide linear range of 5.6×10-5-1.2×10-3 mol/L and a low detection limit of 2.3×10-5 mol/L（at signal/noise ratio S/N = 3）.The modified electrode could be used to detect the real samples with a good stability and repeatability.