大蒜素对慢性肾衰大鼠贫血及血脂、电解质代谢紊乱的影响

Effect of Allicin on Anemia,Blood Fat and Electrolyte Metabolism in Rats with Chronic Renal Failure

目的观察大蒜素对慢性肾衰(CRF)大鼠贫血和血脂、电解质代谢紊乱的影响。方法 100只实验用大鼠随机分为正常对照组、模型对照组、大蒜素[5、10、20 mg/(kg·d)]组,采用腺嘌呤溶液[250 mg/(kg·d)]灌胃21 d的方法建立CRF大鼠模型,大蒜素[5、10、20 mg/(kg·d)]组腹腔注射给药治疗,疗程28 d。测定血清肾功能指标(BUN、SCr、UA含量),计算肾脏指数;测定全血中红细胞(RBC)、血红蛋白(HGB)、血球压积(HCT)、血清铁(SI)、总铁结合力(TIBC)、铁蛋白(FER);测定血浆中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)含量;测定血清中钾(K^+)、钠(Na^+)、钙(Ca^(2+))、磷(P^(3+))含量;HE染色观察肾脏组织病理变化。结果与模型对照组比较,大蒜素10、20 mg/(kg·d)组CRF大鼠血清BUN、SCr、UA含量显著降低(P<0.05或P<0.01),肾脏指数显著降低(P<0.01),HGB、HCT及SI、TIBC、FER均显著升高且20 mg/(kg·d)组RBC显著升高(P<0.05或P<0.01),血浆中TG、TC、LDL-C含量水平显著降低且20 mg/(kg·d)组HDL-C含量水平显著升高(P<0.05或P<0.01),血清中Ca^(2+)含量显著升高(P<0.05),P^(3+)含量显著降低且20 mg/(kg·d)组血清K^+、Na^+显著降低(P<0.05或P<0.01)。结论大蒜素可能通过改善贫血、电解质紊乱及调节血脂而对CRF大鼠起到一定的保护作用。

Objective： To study the effect of allicin on anemia,blood fat and electrolyte metabolism in rats with chronic renal failure（CRF）. Methods： 100 rats were randomly divided into the normal control group,the model control group and allicin[5,10,20 mg/（kg·d）] groups. CRF rat model was established by intragastric administration of adenine solution [250 mg/（kg·d）] for 21 days,and then intraperitoneal injection of allicin [5,10,20 mg/（kg·d）] for treatment,once a day for 28 days. The levels of serum urea nitrogen（BUN）,serum creatinine（SCr）and uric acid（UA） were measured;the kidney index was calculated. The level of red blood cells（RBC）,hemoglobin（HGB）,hematocrit（HCT）,serum iron（SI）,total iron binding capacity（TIBC） and ferritin（FER） in whole blood were measured;the content of triglyceride（TG）,total cholesterol（TC）,low density lipoprotein cholesterol（LDL-C） and high density lipoprotein cholesterol（HDL-C） in plasma were measured;the level of potassium（K~＋）,sodium（Na~＋）,calcium（Ca^（2＋）） and phosphorus（P^（3＋）） in serum were measured. The pathological changes of renal tissue was observed by HE staining. Results： Compared with the model group,the content of BUN,SCr,UA in serum of allicin[10,20 mg/（kg·d）] groups were significantly reduced（P 0.05 or P 0.01）;renal index was significantly reduced（P 0.01）;the level of HGB,HCT,SI,TIBC and FER was significantly increased and RBC of 20 mg/kgod group increased significantly（P 0.05 or P 0.01）;the content of TG,TC and LDL-C in plasma was significantly decreased and the HDL-C in the 20 mg/（kg·d） group was significantly increased（P 0.05 or P 0.01）;the level of Ca^（2＋） in serum was significantly increased（P 0.05） and the level of K~＋,Na~＋ and P^（3＋） were significantly decreased（P 0.05 or P 0.01）. Conclusion： Allicin perhaps has protective effects on CRF rats by improving anemia and electrolyte imbalance and regulating blood lipids.