黄连素对糖尿病合并抑郁症大鼠干预作用的实验研究

The Experimental Study on the Effect of Berberine on Diabetic Rats with Depression

目的:评价黄连素对糖尿病合并抑郁症模型大鼠的干预作用。方法:应用高脂饲料喂养并腹腔注射链脲佐菌素法制备糖尿病大鼠模型,在其基础上进一步应用慢性不可预见性温和应激方法建立糖尿病合并抑郁症大鼠模型。实验动物分为空白组、模型组、盐酸二甲双胍+盐酸氟西汀组、黄连素组,通过口服葡萄糖实验、正糖钳夹试验、悬尾试验、糖水偏好实验以及开野实验,评价黄连素对糖尿病合并抑郁症大鼠的干预作用。结果:黄连素可改善大鼠模型糖代谢及改善胰岛素抵抗,同时改善抑郁症状。结论:黄连素对糖尿病合并抑郁大鼠有较好的作用效果,其机制尚待进一步深入研究。

Objective：To evaluate the effect of berberine on diabetic rats with depression. Methods.. Diabetic rat model with depression was induced by the combination of high-fat diet, injection of Streptozotocin（STZ）, and chronic stress. The model rats were randomly divided into three groups, the DM with depression group, metforrnin combined fluoxetine group,berberine groups. Oral glucose test and euglycemic clamp test were carried out to evaluate rats＇glucose metabo- lism. The depressional behavior was examine using sucrose preference test, tail-suspension test and open field test. Results：Berberine can ameliorate glucose metabolism and insulin resistance in rats, and improve the symptoms of depression. Conclusion： Berberine has a better intervention effect on diabetic rats with depression, and its mechanism needs further study