乳酸杆菌脱氧胞苷激酶的分离纯化和N-末端区序列分析

PURIFICATION AND N-TERMINAL SEQUENCE OF DEOXYCYTIDINE KINASE FROM LACTOBACILLUS ACIDOPHILUS

本文报导目前已知的最小的双功能酶——乳酸杆菌脱氧胞苷激酶/脱氧腺苷激酶(deoxycytidine kinase/deoxyadenosine kinase,即dCK/dAK)的分离纯化,并进行了N—末端区的序列分析。本文还对不同激酶的同源序列进行了比较,证明脱氧胞苷激酶的N—末端区存在ATP结合位点。

One of the smallest bifunctional enzyme—deoxycytidine kinase/deoxyadenosine kinase was purified from Lactobacillus acidophilus. Its N-terminal region has been sequenced. The comparision of homologous sequence about different kinase confirmed that the ATP binding, site of the deoxycytidine kinase is in the N-terminal region.