摘要
以包埋时间、加水量、芯材比、包埋温度为正交实验的因素考察对决明子多糖微囊化的影响。结果显示包埋时间6小时、加水量4 m L、芯材比为3∶1(g/g)和包埋温度40℃,决明子多糖微囊的包埋率为49.3%。HepG2细胞在决明子多糖酵母微囊(100 mg/m L)中的MTT生存率是46.9%,LDH活性是176.9%,且HepG2细胞会皱缩、分离。
The effects of embedding time,water add ition,core ratio and embedding temperature on the microencapsulation of cassia polysaccharide were investigated by orthogonal experiment. The analysis shows that : When the embedding time is 6 hours and the amount of water is 6 mL,the ratio of core to material is 3 ∶1(g/g).Meanwhile,when the embedding temperature is 40 ℃,the embedding rate of cassia polysaccharide microcapsules is 49.3%. Besides,the research shows that the survival rate of HepG2 cells for cassia polysaccharide yeast microcapsules is 46.9%. At the same time,the LDH activity is 176.9% which could cause the shrinkage and separation of the HepG2 cells.
作者
洪自兵
孙洁琼
刘梅
王可
李森
姚向阳
HONG Zibing SUN Jieqiong LIU Mei WANG Ke LI Sen YAO Xianfyang(School of Food and Biology Engineering, Bengbu University, Bengbu Anhui 233030, Chin)
出处
《乐山师范学院学报》
2017年第8期15-19,37,共6页
Journal of Leshan Normal University
基金
大学生创新创业项目"决明子多糖胶囊制备及体外降脂分析"(201511305027)
安徽省教育厅高校自然科学重点项目"蒲公英萜醇调控STAT3通路和自噬功能诱导肿瘤细胞死亡的机制研究"(KJ2014A149)
关键词
决明子多糖
酵母微囊
正交实验
HEPG2细胞
抑制作用
Cassia Polysaccharide
Yeast Microcapsule
Orthogonal Experiment
HepG2 Cell
Inhibiting Effect