荔枝核总黄酮对脓毒症大鼠肠损伤的保护作用

Protection effect of total flavone of Litchi Chinensis Sonn on sepsis-associated intestinal injury in rats

目的探讨荔枝核总黄酮对脓毒症大鼠肠损伤的保护作用。方法将64只Wistar大鼠随机分为假手术组、脓毒症组、荔枝核总黄酮假手术组、荔枝核总黄酮组,每组16只。脓毒症组和荔枝核总黄酮组大鼠均采用腹主动脉阻断术联合腹腔注射脂多糖(LPS)的方法构建严重脓毒症模型;假手术组和荔枝核总黄酮假手术组仅分离腹主动脉但不夹闭,不注射LPS,仅于相应时点腹腔注射等量生理盐水。术后第2天开始,假手术组和脓毒症组给予0.9%氯化钠溶液5 mL/(kg·d)灌胃,荔枝核总黄酮组及荔枝核总黄酮假手术组均给予荔枝核总黄酮150 mg/(kg·d)灌胃,均每天1次,共7 d。各组手术8 h后采血,检测血清中C反应蛋白(CRP)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平;7 d后取小肠组织,检测一氧化氮合酶(NOS)活力及一氧化氮(NO)、超氧化物歧化酶(SOD)、丙二醛(MDA)、谷胱甘肽过氧化物酶(GSH-Px)含量。结果荔枝核总黄酮组大鼠生存率明显高于脓毒症组(P<0.05),但仍低于假手术组(P<0.05)。脓毒症组血清CRP、IL-6、TNF-α水平均明显高于假手术组(P均<0.05),荔枝核总黄酮组血清CRP、IL-6、TNF-α水平均明显低于脓毒症组(P均<0.05)。脓毒症组大鼠肠组织中NO、MDA含量和NOS活力均明显高于假手术组(P均<0.05),SOD和GSH-Px含量均明显低于假手术组(P均<0.05);荔枝核总黄酮组大鼠肠组织中NO、MDA含量和NOS活力均明显低于脓毒症组(P均<0.05),SOD和GSH-Px含量均明显高于脓毒症组(P均<0.05);荔枝核总黄酮假手术组各指标与假手术组比较差异均无统计学意义(P均>0.05)。结论荔枝核总黄酮可以增强机体抗氧化能力,减轻炎症反应及氧化应激损伤,从而达到保护脓毒症肠损伤的目的。

Objective It is to investigate the effect of total flavone of Litchi Chinensis Sonn（TFL） on sepsis-associated intestinal injury in rats. Methods 64 Wistar rats were randomly divided into sham operation group, sepsis group, TFL-sham operation group, TFL group, each group had 16 rats. Sepsis was induced by abdominal aorta clamping combined with intraperitoneal injection of lipopolysaccharide in sepsis group and TFL group, abdominal aorta was separated without clamping and injection of LPS in sham group and TFL-sham group. On the second day after the surgery, sham group and sepsis group were given normal saline 5 mL/（kg · d） by lavage, TFL group and TFL-sham group were given TFL 150 mg（ kg · d） by lavage, once per day, for 7 days. The plasma levels of IL-6, TNF-α and CRP were all determined in 8 h after surgery, the intestinal tissues were obtained to determine the concentration lever of NO, SOD, MDA, GSH-Px and the activity of NOS. Results The 7 d survival rate in TFL group was significantly lower than that in sham group, but higher than that in sepsis group （P 〈 O. 05）. The levels of CRP, IL-6 and TNF-α in sepsis group were higher than those in sham group and TFL group（P 〈 0.05 ）. Compared with the sham group, the levels of NO, NOS and MDA of intestinal tissue increased significantly and SOD and GSH-PX decreased in sepsis group （ P 〈 0.05 ）. The levels of NO, NOS and MDA of intestinal tissue in TFL group were significantly lower but SOD and GSH-PX were higher than those in sepsis group（P 〈 0.05 ） , there was no significant difference between TFL- sham group and sham group （P 〉 0.05 ）. Conclusion TFL might have a protective effect against sepsis-associated intestinal injury through enhancing antioxidant capacity, and reducing the oxidative stress damage and the inflammatory response.