牦牛miR-101的靶基因预测及生物信息学分析

Bioinformatic Analysis and Prediction of miRNA-101 Target Genes in Yak

本研究利用相关生物信息学软件和数据库对牦牛miR-101进行了靶基因预测与生物信息学分析。通过高通量测序获取牦牛miR-101的序列,并分析其序列保守性;选取TargetScan、miRanda、PicTar预测结果的交集并结合miRTarbase中已证实的靶基因集合,取二者并集,采用BINGO和DAVID数据库获得靶基因集合的本体注释,进行GO功能富集及Pathway信号通路富集分析。结果显示,miR-101序列在各物种间高度保守,miR-101调控的靶基因GO功能富集主要包括多细胞生物体发育、发育过程、系统发育、解剖结构发育、器官发育等基本生物学过程和蛋白连接、转录因子活性等分子功能;靶基因存在于细胞各个组分中,包括细胞质、细胞核、细胞器中;信号转导通路显著富集于MAPK信号通路(MAPK signaling pathway)、黏着斑(focal adhesion)、ErbB信号通路(ErbB signaling pathway)、Wnt信号通路(Wnt signaling pathway)、TGF-beta信号通路(TGF-beta signaling pathway)和mTOR信号通路(mTOR signaling pathway)中(P<0.05)。通过对牦牛miR-101靶基因的生物信息学分析,为进一步研究miR-101在牦牛肌肉发育中的作用奠定基础。

In this study,bioinformatics softwares and databases were used to bioinformatically analyze and predict target genes of miR-101 in yak.The high-throughput sequencing technology was used to obtain the sequence of yak miR-101 and analyze its conservation.TargetScan,miRanda and PicTar were applied to predict target genes of miR-101.The intersection of the three results and validated targets from miRTarbase database were demonstrated by Gene Ontology and Pathway enrichment analysis using BINGO and DAVID database.The results showed that miR-101 sequence of yak was highly conserved among all species,and the biological functions of target genes regulated by miR-101 mainly included basic biological function,such as multicellular organismal development,developmental process,system development,anatomical structure development,organ development and molecular functions were enriched in protein binding,transcription factor activity and so on.Target genes exist in various components of the cell,including the cytoplasm,nucleus and organelles.Signal transduction pathways were significantly enriched in the MAPKsignal pathway,focal adhesion,ErbB signaling pathway,Wnt signaling pathway,TGF-beta signaling pathway and mTOR signaling pathway（P〈0.05）.The prediction and bioinformatics analysis of miR-101 target genes laid the basis to study the role of miR-101 in yak muscle development.