艾灸对局灶节段性肾小球硬化大鼠肾脏病理的影响

Effects of Moxibustion on Renal Pathology of Focal Segmental Glomerulosclerosis Rats

目的观察艾灸对局灶节段性肾小球硬化(FSGS)模型大鼠肾脏病理的影响。方法建立FSGS大鼠模型,将大鼠随机分为模型组、氯沙坦组、肾俞灸法各组、膈俞灸法各组,每组11只,另设正常组、假手术组,每组7只。氯沙坦组予氯沙坦灌胃,每日1次;肾俞灸法(短时、中时、长时)组和膈俞灸法(短时、中时、长时)组,分别按不同时长(10 min、20 min、30 min)予温和灸大鼠肾俞、膈俞,隔日1次;假手术组、模型组隔日作与灸法组相同的固定,干预12周。观察各组大鼠肾组织病理变化,分析肾小球硬化指数(GSI)、肾小球毛细血管直径、肾小球系膜基质面积、毛细血管襻横截面积及肾小球系膜基质相对面积。结果模型组、氯沙坦组和灸法各组大鼠肾组织均出现不同程度肾小球硬化。与模型组比较,各治疗组GSI、系膜基质相对面积降低(P<0.01),毛细血管直径、襻横截面积升高(P<0.01),氯沙坦组、膈俞长时组系膜基质面积降低(P<0.05,P<0.01)。结论艾灸可改善FSGS大鼠病理,抑制细胞外基质聚积,延缓肾小球硬化进展。

Objective To observe the effect of moxibustion on the renal pathology in model rats of focal segmental glomerulosclerosis（ FSGS）. Methods FSGS rat model was established and randomly divided into the model group,the losartan group,the BL-23（ Shenshu） moxibustion group and the BL-17（ Geshu） moxibustion group,11 in each group. The normal group and sham group were also setup. The losartan group was intragastrically administered with losartan once daily while the BL-23 and the BL-17 moxibustion group were treated with moxibustion according to the different duration（ 10 min,20 min,or 30 min per acupoint） every other day. The sham group and the model group were fixed as the moxibustion group once every two days. After 12 weeks,the renal pathological changes were observed,glomerulosclerosis index（ GSI）,capillary diameter,area of mesangial matrix,cross-sectional area of capillary loop,and relative area of mesangial matrix were analyzed. Results The kidney tissue of rats in the model group,the losartan group and the moxibustion group showed varying degrees of glomerular sclerosis. Compared with the model group,GSI and relative area of mesangial matrix decreased,capillary diameter and cross-sectional area of capillary loop increased in the treatment groups（P 0. 01）; the area of mesangial matrix decreased in the losartan group and the BL-17（ 30 min） group（P 0. 05,P 0. 01）. Conclusion Moxibustion could improve the pathological changes of FSGS rats model,inhibit or prevent the extracellular matrix accumulation and glomerulosclerosis.