组合处理对双菌种生物被膜的清除和杀灭效果

Removal effect and killing efficacy of combination treatments on dual-species biofilms

该研究建立了食源性病原菌(液化沙雷菌S1和腐生葡萄球菌S2)双菌种生物被膜研究模型,采用微孔板法和MTT法检测单一、组合处理方式对双菌种生物被膜的清除和杀灭效果。研究发现,采用24孔板37℃于TSB培养基中培养24 h可获得稳定的液化沙雷菌-腐生葡萄球菌双菌种生物被膜,组合处理方式优于单一处理效果,处理顺序的不同也会影响生物被膜的清除和杀灭效果,1%Na OH处理20 min再用0.3%Na Cl O处理20 min清除效果最好,双菌种生物被膜的量由1.11(OD595 nm)减少至0.08(OD595 nm),杀灭率达95.73%。

The study mainly focused on the establishment of a dual-species biofilm model formed by two foodbome pathogenic bacteria Serratia lique- faciens S1 and Staphylococcus saprophyticus S2. Microtitor-plate method and MTT assay were used to measure the removal effect and killing effica- cy of single or combination treatments on dual-species biofilm formation. The results showed that the stable dual-species biofilms, consisting of S. liquefaciens S1 and S. saprophyticus S2, could be obtained in tryptone soy broth （TSB） at 37 ℃ for 24 h. The effects of combined treatments were better than single treatments. Moreover, in combined treatments, the removal effect and killing efficacy were also influenced by treatment orders. Results indicated that when the dual-species biofilms were treated with 1% NaOH for 20 min firstly and then disposed with 0.3% NaC10 for another 20 min, the removal effects of biofilms were better than that under other conditions, while the dual-species biofilms quantity decreased from 1.11 （OD595MM） to 0.08 （OD595MM） and the killing efficacy reached to 95.73%.