白砂糖中出芽短梗霉的鉴定及溯源

Identification and tracing of Aureobasidium pullulans in white granulated sugar

在制糖干燥包装车间中找到白砂糖出芽短梗霉(Aureobasidium pullulans)的污染来源,建立白砂糖污染微生物的溯源方法。采用传统形态学、聚合酶链式反应(PCR)技术和基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)技术结合的溯源方法,在制糖干燥包装车间的生产线上进行样品污染菌出芽短梗霉的溯源。在车间的白砂糖、通风口空气、排风口空气、鼓风机表面、干燥机表面等均分离出出芽短梗霉,这几株菌的特异性DNA序列和蛋白质指纹图谱相似度极高。白砂糖与车间环境之间存在交叉污染,需加强该车间的清洁及消毒灭菌工作,需在该车间的通风口和排放口配置高效过滤网。构建以传统形态学、PCR技术和MALDI-TOF MS技术结合的白砂糖微生物溯源方法,为制糖企业有针对性地采取有效控制措施提供实验基础和理论依据。

The pollution source of Aureobasidium pullulans in white granulated sugar was found out in the dry packing workshop, and a tracing method of microorganism causing white granulated sugar pollution was established. Combining with traditional morphology, PCR technique and MALDI-TOFMS technique, the tracing of A. pullulans on samples was conducted to the production line of dry packing workshop of sugar refinery. A. pullulans was screened from the white granulated sugar in workshop, ventilation opening air, exhaust outlet air, surface of air blower and surface of drying machine, etc. The similarity of specific DNA sequence and protein finger-print of these strains was extremely high. There was cross con- lamination between white granulated sugar and workshop environment, so it needs to strengthen the cleaning and sterilization of the workshop. In ad- dition, hepa filter should be set in the ventilation opening and discharge outlet of the workshop. In this paper, the microorganism tracing method of white granulated sugar combined with traditional morphology, PCR technique and MALDI-TOFMS technique was established, to provide experi- mental foundation and theoretical basis for taking effective control measures of sugar enterprises.