新疆阿勒泰地区酸奶疙瘩中乳酸菌的分离和鉴定

Isolation and identification of lactic acid bacteria in yogurt pimple from Aletai prefecture of Xinjiang

从新疆阿勒泰地区牧民制作的传统酸奶疙瘩中分离、筛选出菌株SA,通过过氧化氢酶试验阴性初步确定其为乳酸菌,对菌株SA进行菌落和细胞形态、生理生化特征及16S r DNA基因序列分析及系统进化树分析。结果表明,分离菌株SA与Weissella cibaria KACC 11862 AEKT01000037聚为一类,其同源性为99%,故将其鉴定为食窦魏斯氏菌(Weissella cibaria)。其最佳生长条件为40℃、p H 6.0,并且能够耐受3%的Na Cl。

Strain SA isolated and screened in yogurt pimple produced by herdsmen from Aletai prefecture of Xinjiang, was preliminarily determined as a lactic acid bacterium by catalase tests. Strain SA was researched by colony and cell morphology, physiological and biochemical characteristics, 16S rDNA gene sequences and phylogenetic tree analysis. The results showed that isolated strain SA was clustered into one class with Weissella cibaria KACC 11862 AEKT01000037, and the homology was 99%. Strain SA was identified as Weissella cibaria. The optimum growth conditions of strain SA was culture temperature 40℃ and pH 6.0, it could tolerate 3% NaC1.