鲜剥大蒜中腐败微生物的分离纯化及鉴定

Isolation, purification and identification of spoilage microorganisms in fresh garlic

对鲜剥大蒜霉变部分的腐败微生物进行分离纯化,观察待测菌株的菌落形态特征、菌丝显微结构,并进行分子生物学分析。结果表明,经过分离纯化后一共得到4株菌,通过对其菌落形态特征和菌丝显微结构观察,初步判定为青霉、根霉、赤霉及曲霉;通过聚合酶链式反应(PCR)扩增、产物测序和序列分析,最终鉴定4株菌分别为:桔青霉(Penicillium citrinum)、匍枝根霉(Rhizopus stolonifer)、赤霉(Gibberella intermedia)、赭曲霉(Aspergillus ochraceus)。

Spoilage microorganisms derived from the moldy part of fresh garlic were isolated and purified, and the morphological characters, the mycelial microstructure and molecular biological analysis of the strains were analyzed. The results indicated that there were 4 strains screened after isolation and purification, and they were preliminarily identified as Penicillium, Rhizopus, Gibberella and Aspergillus by observing the morphological characters and mycelial microstructure. After PCR amplification, product sequencing and sequence analysis, the strains were identified as Penicillium citrinum, Rhizopus stolonifer, Gibberella intennedia and Aspergillus ochraceus.