亚临界提取蜜桔皮精油成分分析及其微乳液制备

Analysis of essential oil extracted by subcritical fluid extraction from honeyorange peel and preparation of microemulsion

采用亚临界流体萃取法提取宜昌蜜桔皮精油,用顶空固相微萃取—气相色谱—质谱联用技术(SPME-GC-MS)分析蜜桔皮精油中挥发性成分及脂肪酸组成,用高速分散及超高压均质制备O/W型蜜桔皮精油微乳液,确定高稳定性微乳液的乳化剂最适用量、最适油水比和最佳均质条件。结果表明:亚临界流体萃取蜜桔皮精油,平均得率约1%。精油挥发性成分主要由烃类和酸类化合物组成,分别占总量的58.68%和40.34%;宜昌蜜桔皮精油脂肪酸中主要含不饱和脂肪酸,达77.35%,其中亚油酸含量占总脂肪酸含量的37.80%,饱和脂肪酸主要为棕榈酸,含量为14.18%。均一微乳液制备的最适乳化剂SE-15质量分数为6%,最适油水质量比为1:25,最佳均质条件为:压力50 MPa,均质4次。

The essential oils of Yichang honeyorange peel were extracted by subcritical fluid extraction,and the headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry（SPME-GC-MS）method was applied to analysis the volatile components and fatty acids.O/W-type essential oil microemulsion was prepared by using SE-15 as emulsifier to determine the optimum amount of emulsifier,optimum ratio of oil-to-water and optimum homogenization condition of emulsifier for stable microemulsion.The results showed that the yield of essential oil was 1% by subcritical fluid extraction.And the volatile components in essential oil were mainly composed of hydrocarbons compounds and acid compounds,accounting for 58.68% and 40.34% of the total,respectively.GC-MS analysis of fatty acids showed that the essential oils mainly include unsaturated fatty acids,which account for 77.35%.linoleic acid account for 37.80% of the total.Saturated fatty acid mainly included palmitic acid,accounting for 14.18%.To prepare the homogeneous microemulsion,the optimum emulsifier content is 6%,the optimum quality ratio of oil to water is 1：25,the optimum homogenization condition is：pressure 50 MPa,the number is 4times.