摘要
目的观察不同时间脉冲射频(PRF)作用于慢性坐骨神经压迫损伤(CCI)大鼠模型下丘脑β-内啡肽含量的影响。方法雄性SD大鼠72只,随机分成4组:假PRF治疗组(A0组),120sPRF治疗组(A1组),480sPRF治疗组(A2组),720SPRF治疗组(A3组),每组18例。建模后7d,对PRF组坐骨神经结扎处近端进行PRF干预治疗,脉宽20ms,脉冲频率2Hz,电压55v,治疗持续时间分别为120s,480S,720s;假PRF治疗组,在PRF治疗组坐骨神经相同位置处只放置射频电极针,但不给予通电治疗。PRF治疗后7、14、21d时,每组各时间点分别处死6只大鼠,取下下丘脑,用酶联免疫法测定不同时间点下丘脑中β-内啡肽的浓度,光镜下观察大鼠坐骨神经组织学变化。结果PRF治疗组在治疗后7、14d时,与假PRF治疗组比较大鼠下丘脑β-内啡肽含量明显升高(P〈0.01)。治疗后14d时,A3组和A2组明显高于A1组(P〈0.05);治疗后21d时,A3组高于A2组(P〈0.05),A2组高于A1组(P〈0.05)。在光镜观察下与假PRF治疗组比较,经PRF干预后的坐骨神经结构变化轻微,而且随着时间延长呈恢复趋势。结论作用时间为120、480、720S的脉冲射频均可引起CCI大鼠下丘脑β-内啡肽含量明显升高。适当增加脉冲射频的作用时间,会在更长时间内使CCI大鼠下丘脑的β-内啡肽维持较高的浓度,可能延长脉冲射频镇痛时间。
Objective To observe the effects of pulsed radiofrequency with different treatment times on the beta-endorphin in hypothalamus in a rat model of chronic neuropathie pain. Methods Seventy-two male SD rats weighing 200-220 g were randomly divided into 4 groups (n=18 in each group): sham PRF group (A0), PRF 120 s group (A1), PRF 480 s group (A2), PRF 720 s group (A3). Neuropathic pain was induced by chronic constrictive injury (CCI). PRF intervention was performed on the proximal part of the sciatic nerve ligation in group PRF at 7 d after CCI, with pulse duration 20 ms, pulse frequency 2 Hz, voltage 55 V, and the duration of treatment was 120 s, 480 s, 720 s respectively. The radiofrequency electrodes were placed at the same position of the sciatic nerve without electricity applied in sham PRF group. The expression of beta-endorphin by enzyme-linked immunosorbent assay (ELISA) was respectively measured in the hypothalamus in each group (n=6) at 7, 14, 21 d after PRF treatment and the histological changes of the sciatic nerve in rats were observed under light microscope. Results The expression of beta-endorphin in the hypothalamus of the rats was significantly higher in the PRF group than that in sham PRF group (P〈0.01) at 7, 14 d after PRF treatment. The expression of beta-endorphin was significantly higher in PRF 720 s group and PRF 480 s group than that in PRF 120 s group at 14 d after PRF treatment (P〈0.05), which was higher in PRF 720 s group than that in PRF 480 s group, higher in PRF 480 s group than that in PRF 120 s group at 21 d after PRF treatment (P〈0.05). The sciatic nerve structure changed slightly after PRF treatment, and showed a trend of recovery over time under light microscope. Conclusion The content of beta-endorphin in hypothalamus of CCI rats is significantly increased after PRF treatment with 120 s, 480 s and 720 s. Appropriately increasing the time of pulsed radiofrequency will maintain high concentration of beta-endorphin in hypothalamus of CCI rats for a long time, and may prolong the time of PRF analgesia.
作者
宋波
薛朝霞
杨保仲
耿宝梁
王晓东
Song Bo Xue Zhaoxia Yang Baozhong Geng Baoliang Wang Xiaodong(Department of Anesthesiology, Shanxi Medical University, Taiyuan City, Shanxi Province 030001, China)
出处
《实用疼痛学杂志》
2017年第4期243-248,共6页
Pain Clinic Journal
关键词
脉冲射频
大鼠
神经痛
Β-内啡肽
光镜
Pulsed radiofrequency
Rat
Neuropathic pain
Beat-endorphin
Optical micro- scope