马黛通络饮对APOE基因敲除小鼠脂质代谢的影响

Effects of Madai Tongluo decoction on lipid metabolism in apolipoprotein E-deficient mice

目的观察马黛通络饮对载脂蛋白E基因敲除小鼠脂质代谢的作用机制。方法 64只5周龄ApoE-/-小鼠高脂饲料喂养,马黛通络饮灌胃干预8周后,检测小鼠血脂含量及主动脉的酯酰辅酶A-胆固醇酰基转移酶1(acy1-coenzyme A-cholesterol acyltransferases1,ACAT1)、氧化低密度脂蛋白的内皮受体(lectin-like OX-LDL receptor 1,LOX-1)的蛋白表达。结果模型组血液中的总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)浓度明显高于正常组。与模型组相比较,马黛通络饮高剂量组明显降低血清TC、TG、LDL-C水平及主动脉组织中的ACAT1、LOX-1的表达,明显升高血清高密度脂蛋白胆固醇(highdensity lipoprotein cholesterol,HDL-C)水平(P<0.05),低剂量组对TG、LDL-C、HDL-C的作用效果显著(P<0.05),而对TC及ACAT1、LOX-1作用效果不明显(P>0.05)。结论马黛通络饮能降低ApoE-/-小鼠血脂水平,抑制ApoE-/-小鼠主动脉组织中的ACAT1、LOX-1的表达,通过调节血脂异常和脂质沉积发挥其阻止动脉粥样硬化及心脑血管疾病发生和发展的作用。

Objective To study the effect mechanism of Madai Tongluo decoction on vascular en- dothelial injury of atherosclerosis in apolipoprotein e-knockout mice. Methods Five-week-old male ApoE -/- mice were fed with a high fat high cholesterol diet. $ weeks after the intervention of Madal Tongluo decoction. The level of serum lipid and the expression of acyl-coenzyme A-cholesterol acyltransferasesl （ ACATI ）, Lectin-like OX-LDL receptor 1 （ LOX-1 ） was detected. Results Serum contents of TC, TG and LDL-C in the Model group were significantly higher than that in normal group. Serum contents of TC, TG, LDL-C and the ACATI ,LOX-1 of aortic tissue in Madai Tongluo decoction high-dose group were sig- nificantly lower than those in model group （P〈0.05）, and serum HDL-C in Madai Tongluo decoction high-dose group was significmitly lower than that in model group. TG,LDL-C ,HDL-C and LOX-1 in Madai Tongluo decoction low-dose group were significant improved （P〈0.05） , but TC and ACAT1 were non- significant（P〉O. 05 ）. Conclusions Madai Tongluo decoction can regulate lipid metabolism of ApoE -/- mice and inhibit ACAT1 and LOX-1 expression of aortic tissue. Madai Tongluo decoction can inhibit the occurrence and development of atherosclerotic lesions, cardiovascular and cerebrovascular diseases, the possible mechanisms may be associated with lipid metabolism and inhibition of lipid deposition.