银杏叶提取物对肠炎小鼠肠黏膜屏障的保护作用研究

Protective role of Gingko Biloba extract in intestinal mucosal barrier of intestinal inflammation mouse

目的探索银杏叶提取物(EGB 761)对肠炎模型小鼠结肠黏膜屏障的保护作用。方法将30只小鼠分为正常对照组、模型组、EGB 761组,建立动物模型;每天观察小鼠的一般情况、体质量变化、粪便隐血及结肠组织病理学的表现,免疫组织化学荧光染色测定结肠组织中occludin-1等紧密连接蛋白的变化。结果模型组小鼠体质量下降,饮水和进食明显减少,EGB761干预治疗后小鼠的体质量回升明显;EGB 761组可见炎症细胞浸润少,炎症程度较模型组明显减轻。模型组结肠黏膜组织紧密连接蛋白-1(claudin-1)、occludin和zo-1荧光分布较正常对照组分散,荧光强度减弱,边缘粗糙;经EGB 761干预后EGB 761组荧光仍沿细胞膜分布,强度较正常对照组稍减弱,但仍强于模型组。结论 EGB 761能有改善肠炎模型小鼠结肠组织炎性反应,其机制可能与加强其肠黏膜屏障的保护作用有关。

Objective To investigate the protective role of Gingko Biloba extract （EGB 761） in intestinal mucosal barrier of intestinal inflammation model mouse. Methods Thirty mice were randomly divided into the control group, model group and EGB 761 group;the animal model was established;the general condition,body mass change, fecal occult blood and colon histopathological changes were observed, the expressions of occludin-1 proteins in colon tissue were detected by immunofiuorescence. Results The body mass in the model group appeared to decrease, drinking water and eating food were significantly decreased compared with that in the control group. However after the EGB 761 intervention, the body mass of the model mice was significantly risen again; little inflammatory cells infiltration could beseen in the.EGB 761 group, the inflammatory degree was significantly alleviated compared with the model group. The fluorescence distribution of the elaudin-1 ,occludin and zo-1 in colonic tissues of the model group was more disperse compared with the normal group,the fluorescence intensity was weakened with rough edge ;after EGB 761 intervention, the fluorescence in the EGB7 61 group was distributed along with the cellular membrane, the intensity was slightly weakened compared with the normal control group,but was still stronger than that in the model group. Conclusion EGB 761 can improve the inflammatory reaction in mouse colonic tissue,its mechanism may be related with its strengthening the protective effect of intestinal mucosal barrier.