高原低氧致应激性溃疡大鼠模型的建立

Establishment of rat model of plateau hypoxia caused stress ulcer

目的建立大鼠高原低氧致应激性溃疡实验动物模型。方法将大鼠分为6组:常氧组、高原低氧2d组、高原低氧4d组、高原低氧6d组、高原低氧8d组和高原低氧10d组。除常氧组外,其余各组大鼠分别在低压氧舱内(模拟海拔5 000m)饲养2、4、6、8d和10d。观察各组大鼠胃黏膜溃疡指数、胃液量、胃蛋白酶活性和胃液pH值的变化。结果大鼠在低压氧舱内模拟海拔5 000m高原低氧2、4、6、8、10d后,均可见胃黏膜多个不同程度的点状和线状出血、糜烂及溃疡形成;溃疡指数明显增加;胃液量和pH值均明显下降,胃蛋白酶活性明显升高。各指标变化均在高原低氧第6天时最明显。结论大鼠在模拟海拔5 000m的低压氧舱内饲养2~10d,均可建立高原低氧致应激性溃疡动物模型,且最佳时间是6d。

Objective To establish the rat experimental animal model of plateau hypoxia caused stress ulcer. Methods Rats were randomly divided into the normoxia group, plateau hypoxia 2,4,6,8,10 d groups. Except the norrnoxia group, the rats in other groups were fed in a hypobaric chamber（ simulated altitude 5000 m） for 2,4,6,8,10 d srespectively. Then the gastric mucosal ulcer index,gastric juice volume,pepsin activity and PH value of gastric juice were observed in each group. Results Different degrees of point-like and line-like bleeding,erosion and ulcer formation could be seen in rat gastric rnucosa in the hypobaric chamber（ simulated altitude 5 000 m） on 2,4,6,8,10 d of plateau hypoxia, the ulcer index was significantly increased ; the PH value and gastric juice volume were significantly decreased and the pepsin activity was significantly elevated. The changes of various indexes were most obvious on 6 d of plateau hypoxia. Conclusion Rats fed in a hypobaric chamber simulating a altitude of 5 000 m for 2 - 10 d can eastablish the altitude hypoxia caused stress ulcer animal model,moreover the optimal time is 6 d.