云南省宣威地区非小细胞肺癌中石英沉积与核因子κB表达的相关性分析

Correlation analysis between expression of NF-κB and quartz＇s deposit in non-small cell lung cancer in Xuanwei , Yunnan province

目的 探讨云南省宣威地区非小细胞肺癌（NSCLC）组织中石英沉积与核因子κB（NF-κB）的表达关系,评价石英沉积在宣威地区NSCLC致癌机制中的作用.方法 收集2009年7月至2015年9月昆明医科大学第三附属医院手术切除的NSCLC及良性（或炎性）病变肺组织标本.肺组织病理标本行透射电镜（TEM）观察,选区电子衍射（SAED）及X线能量色散分析（EDS）,观测各组中石英沉积和局部病理变化.检测肺组织中NF-κB蛋白的表达,与沉积石英粒径行相关分析.结果 TEM结果显示宣威地区NSCLC组肺组织中的石英赋存率明显高于非宣威地区NSCLC组及良性病变组（P〈0.01）,其石英平均粒径为（226±120）nm×（237±163）nm,小于后两组中的赋存粒径（P〈0.05）;宣威地区NSCLC组肺组织中NF-κB蛋白表达水平明显高于非宣威地区NSCLC组及良性病变组（P〈0.01）,其癌组织与正常肺组织中的表达水平差异无统计学意义（P〉0.05）;样本肺组织中的石英粒径与NF-κB蛋白表达水平呈负相关趋势.结论 石英沉积在宣威地区NSCLC的致癌机制中发挥一定作用,粒径越小细胞毒性越大.

Objective To evaluate the relationship between quartz＇s deposit and expression of NF-κB in non-small cell lung cancer （NSCLC） lung tissues in Xuanwei, Yunnan Province, and to clarify the role of quartz in Xuanwei NSCLC＇s carcinogenic mechanism. Methods As research objects, the lung tissues of NSCLC and lung benign lesions after surgical resection were collected from July 2009 to September 2015 at the Third Affiliated Hospital of Kunming Medical University. Firstly, the transmission electron microscopic （TEM） with energy dispersive X-ray analyzer （EDS） was used for observation of crystalline deposit and local pathological changes. Secondly, expression level of NF-κB had been analysed and a correlation analysis with particle size of SiO2 crystal in the same lung sample was made. Results The occurrence rates of quartz in Xuanwei NSCLC lung tissues were above non-Xuanwei NSCLC and benign lung tissues （P〈0.01）;the average particle size of SiO2 crystal was （226 ± 120） nm ×（237 ± 163） nm in Xuanwei NSCLC group and it was smaller than the other two groups; In Xuanwei NSCLC group, the expression level of NF-κB was significantly higher than non-Xuanwei and benign lung tissues （P〈0.01）, but there was no significant difference between cancer tissues and normal lung tissues in the group （P〉0.05）. The expression level of NF-κB was generally increasing when quartz ＇s size became smaller. Conclusion Quartz ＇s deposit may play a certain role in carcinogenic mechanism of lung cancer in Xuanwei, the smaller the particle size, the greater the cytotoxicity.