ZKSCAN3在乳腺癌组织中的表达及其临床意义

Expression of ZKSCAN3 in breast cancer tissues and its significance

目的:探讨ZKSCAN3(zinc finger with KRAB and SCAN domains 3)在乳腺癌组织中的表达及其临床意义。方法 :应用免疫组织化学法检测120例乳腺癌组织及60例癌旁组织中ZKSCAN3蛋白的表达,并分析其与患者临床病理参数和总生存率之间的关系。进一步应用实时荧光定量PCR法和蛋白质印迹法检测乳腺癌组织及其相应癌旁组织中ZKSCAN3 m RNA和蛋白的表达水平。结果:乳腺癌组织中ZKSCAN3蛋白的阳性表达率[74%(89/120)]高于相应的癌旁组织[37%(22/60)](P<0.001)。ZKSCAN3蛋白的表达与乳腺癌患者肿块大小、淋巴结转移、分化程度和肿瘤分期相关(P值均<0.05),ZKSCAN3阳性表达患者的总生存率明显低于阴性患者(P=0.040)。乳腺癌组织中ZKSCAN3 m RNA和蛋白的表达水平均高于癌旁组织(P值均<0.01)。结论 :ZKSCAN3与乳腺癌的发生、进展及转移有关,可能是乳腺癌的促癌基因。

Objective： To investigate the expression of zinc finger with KRAB and SCAN domains 3（ZKSCAN3） in breast cancer tissues and its clinical significance.Methods： The expression of ZKSCAN3 in 120 specimens of human breast cancer tissues and 60 specimens of adjacent para-cancerous tissues was detected by immunohistochemistry, and its correlation with clinicopathological parameters and overall survival was analyzed. The real-time fluorescent quantitative PCR and Western blotting were used to further detect the expressions of ZKSCAN3 m RNA and proteinin breast cancer tissues and the adjacent para-cancerous tissues.Results： The positive expression rate of ZKSCAN3 protein in breast cancer tissues [74%（89/120）] was higher than that in the adjacent para-cancerous tissues [37%（22/60）]（P 〈0.001）. The expression of ZKSCAN3 protein was correlated with tumor size, lymph node metastasis, tumor differentiation and clinical stage of breast cancer（all P 〈0.05）. The overall survival rate of patient with ZKSCAN3-positive breast cancer was significantly lower than that of patient with ZKSCAN3-negative breast cancer（P = 0.040）. The expression levels of ZKSCAN3 m RNA and protein in breast cancer tissues were higher than those in the adjacent para-cancerous tissues（both P〈 0.01）.Conclusion： ZKSCAN 3 is associated with the occurrence, progression and metastasis of breast cancer, so it may be an oncogene of breast cancer.