子宫颈癌中SIX1的表达及其与细胞增殖的关系

Expression of SIX1 in cervical cancer and relationship with cervical cancer cell proliferation

目的探讨SIX1在子宫颈癌组织中的表达以及生物学作用。方法采用免疫组化Eli Vision两步法检测35例子宫颈癌、42例子宫颈上皮内病变(cervical intraepithelial neoplasia,CIN)及15例正常子宫颈组织中SIX1的表达及Ki-67增殖指数。应用RNA干扰技术,采用SIX1-siRNA表达质粒转染子宫颈癌Hela细胞,检测转染后子宫颈癌Hela细胞SIX1的mRNA和蛋白表达水平;应用MTT检测Hela细胞的增殖能力。结果子宫颈癌组织中SIX1阳性率高于CIN组织及正常子宫颈组织(P<0.05),且与Ki-67增殖指数呈正相关(P<0.05)。RT-PCR和Western blot检测显示转染SIX1-siRNA子宫颈癌Hela细胞中SIX1在mRNA和蛋白水平均显著下调;Hela细胞生长速度减慢。结论 SIX1可能通过促进子宫颈癌细胞的增殖,在子宫颈癌的发生、发展过程中发挥重要作用。

Purpose To investigate the expression of SIX1 in cervical cancer and its biological function. Methods The expression of SIX1 and Ki-67 in 35 cervical cancer tissues, 42 cervical intraepithelial neoplasia （CIN） tissues and 15 normal cervical tissues was detected by immunohistochemical of EliVision two-step staining. A plasmid vector expressing SIX1 siRNA was constructed and then stably transfected into Hela cells. The expression of SIX1 mRNA and protein was examined by RT-PCR and Western blot method respectively. The proliferation was ex- amined by MTT. Results The positive rate of SIX1 in cervical cancer was significantly higher than that of CIN and normal cervical tissues and was positively correlation with the proliferation index of Ki-67. After RNAi treatment, the mRNA and protein levels of SIX1 was down-regulated in Hela cells and the proliferation of Hela ceils was inhibited. Conclusion SIX1 may play an important role in the occurrence and development process of cervical cancer by promoting cervical cancer cell proliferation.