鼻窍通颗粒中黄芪、黄芩的薄层鉴别研究

Studies on thin layer chromatography identification of Astragalus Radix and Scutellariae Radix in Biqiaotong granules

目的建立医院制剂鼻窍通颗粒中黄芪、黄芩的质量控制方法,为质量标准的制定提供依据。方法采用单因素试验方法,对影响黄芪、黄芩的薄层色谱因素(包括不同的展开系统、显色剂、温度、湿度、点样量和不同厂家薄层板)进行系统考察,筛选出最佳的薄层色谱条件。结果黄芪采用硅胶G薄层板,以三氯甲烷-甲醇-水(13∶7∶2)的下层溶液为展开剂,取出,晾干,喷以10%硫酸乙醇溶液,在105℃加热至斑点显色清晰;黄芩采用硅胶G薄层板,以甲苯-乙酸乙酯-甲酸-甲醇(9∶3∶2∶2)为展开剂,取出,晾干,日光下放置30 min后检视斑点显色清晰。结论薄层色谱特征斑点清晰、分离效果好,专属性强,重复性好,阴性对照品无干扰。该方法简单方便、准确灵敏,可有效控制鼻窍通颗粒的质量。

Objective To establish a method for quality control of Astragalus Radix and Scutellariae Radix in Biqiaotong granules and provide basis for the establishment of quality standard. Methods The single-factor test method was used to investigate the factors of thin layer chromatography （TLC） conditions, including different extract method and solvents, developing system, comogemc agents, temperature, humidity, drawing amounts and thin layer boards, and to screen the best TLC conditions of Astragalus Radix and Scutellariae Radix. Results The TLC conditions of Astragalus Radix were used trichloromethane-methanel-water （13：7：2） as developing solvent, separated on silica gel G, heatd under 105 ~C until the spots bacame clear. The TLC conditions of Scutellariae Radix were used methylbenzene-ethy acetate- formic acid-methanel （9：3：2：2） as developing so/vent, separated on silica gel G, observed after 30 minutes under daylight until the spots were dear. Conclusions The spot features are clear, and with good separating degree, strong specificity, and good repeatability without the inference of negative control. The TLC method is simple, sensitive and accurate, which can be adopted for the quality control of Biqiaotong granules.