培哚普利对心肌肥厚大鼠中碱性成纤维细胞生长因子的影响

Effects of perindopril on basic fibroblast growth factor in rats with myocardial hypertrophy

目的观察培哚普利对心肌肥厚大鼠传导通路中的碱性成纤维细胞生长因子(b FGF)的作用机制。方法将90只雄性健康SD大鼠随机分为模型组、对照组和实验组,每组各30只。模型组皮下注射异丙肾上腺素3 mg·kg^(-1)·d^(-1)与0.9%Na Cl 2m L灌胃;对照组经皮下注射等剂量0.9%Na Cl与0.9%Na Cl2 m L灌胃;实验组经皮下注射异丙肾上腺素3 mg·kg^(-1)·d^(-1)与培哚普利1 mg·kg^(-1)·d^(-1)溶于0.9%Na Cl 2 m L灌胃。每天1次,共10 d。给药后,测量并计算左心室和心脏的质量指数,观察经苏木精-伊红(HE)染色的左心室心肌组织形态和排列情况,用荧光定量聚合酶链式反应(RT-PCR)检测b FGF mRNA在左心室心肌组织的表达情况。结果实验组、模型组和对照组的左心室质量指数分别为(1.95±0.11),(2.47±0.09),(1.45±0.11)g·m^(-2);心脏质量指数分别为(2.69±0.36),(3.81±0.10),(1.92±0.12)g·m^(-2);心肌细胞中的b FGF mRNA相对表达量分别为0.64±0.11,0.74±0.11,0.38±0.08,实验组分别与对照组和模型组比较,差异均有统计学意义(均P<0.05)。结论培哚普利能降低b FGF表达量,从而抑制心室的重构。

Objective To observe the mechanism of perindopril on basic fibroblast growth factor（ bFGF） in the conduction pathway of cardiac hypertrophy rats. Methods A total of 90 male healthy SD rats were randomly divided into model group,control group and experimental group.The model group were subcutaneously injected with 3 mg·kg^（-1）·d^（-1）of isoproterenol,and intragastric administration of saline 2 mL. Control group was injected with saline by hypodermic injection,and intragastric administration of saline 2 mL. Experimental group were subcutaneous injection with 3 mL·kg^（-1）·d^（-1）of isoproterenol,and 2 mL intragastric administration of 1 mg · kg^（-1）·d^（-1）perindopril in saline once daily,each group was treated for 10 days. After treatment,the left ventricular mass and heart mass index were measured and calculated,and the morphology and arrangement of left ventricular myocardium were observed by hematoxylin-eosin（ HE） staining. The expression of b FGF mRNA in left ventricular myocardium was detected by fluorescent quantitative PCR（ RT-PCR）. Results The left ventricular mass indexes in the experimental group, the model group and the control group were（ 1. 95 ± 0. 11）,（ 2. 47 ± 0. 09）,（ 1. 45 ± 0. 11） g·m^（-2）; the heart mass index were（ 2. 69 ± 0. 36）,（ 3. 81 ± 0. 10）,（ 1. 92 ± 0. 12） g · m^（-2）; the relative mRNA expression levels of b FGF in cardiomyocytes were 0. 64 ± 0. 11,0. 74 ± 0. 11,0. 38 ± 0. 08 respectively. There were significant differences between the experimental group and the control group as well as the model group（ P〈0. 05）. Conclusion Perindopril can reduce the expression of b FGF and thus inhibit ventricular remodeling.