β-环糊精-对氯苯基氨基甲酸酯手性固定相的色谱行为

Chromatographic behavior of β-Cyclodextrin-p-chlorophenylcarbamate chiral stationary phases

合成了β-环糊精-对氯苯基氨基甲酸酯衍生物,并分别涂敷于氨丙基硅胶和硅胶表面,制得两种涂敷型β-环糊精-对氯苯基氨基甲酸酯手性固定相(CSP1,CSP2),并以!-异氰酸酯丙基三乙氧基硅烷为连接剂将此衍生物键合于硅胶表面制得同类型键合手性固定相(CSP3)。自制CSPs具有较好的手性识别能力。CSP3在多模式流动相组成下对21个手性样品呈现出不同的手性分离能力。此外,CSP2和CSP3对硝基苯酚的邻、间、对位置异构体也表现出很好的分离能力(分离度R>4)。

The β-cyclodextrin-p-chlorophenylcarbamate derivative was prepared. Two β-cyclodextrinp-chlorophenylcarbamate chiral stationary phase（ β-CD-CSPs,CSP1 and CSP2） were prepared by coating the derivative on aminopropyl silica gel and silica gel, respectively. Bonded CSP3 was synthesized using γ-（ triethoxysilyl） propyl isoyanate as the bonding reagents and silica gel as support. The results showed that the 3 β-CD CSPs exhibit better enantioseparation. CSP3 showed different enantioseparation characteristic for 21 racemates under multimodal elution. Moreover,CSP2 and CSP3 exhibit best separation ability for positional isomers of nitrophenol.