基于铁(Ⅲ)-方酸螯合体系的葡萄糖光谱分析

Spectral analysis for trace glucose measurement based on the chelating system of Fe^(3+)-squaric acid

建立了一种基于Fe^(3+)-方酸螯合体系的葡萄糖可见光谱分析方法。在葡萄糖氧化酶(GOx)的催化作用下,葡萄糖被氧化为葡萄糖酸和H_2O_2,产生的H_2O_2将Fe^(2+)氧化为Fe^(3+),Fe^(3+)与方酸的螯合体系可产生灵敏的显色反应,从而建立了葡萄糖的检测方法。结果表明,所构建的葡萄糖传感器,葡萄糖在0.01~1.0 mmol/L的浓度范围内有很好的线性关系(R=0.9962),检测限为5μmol/L。与市售血糖仪相比,表现出更高的灵敏度,且在8 h内有很好的稳定性。方法可用于胎牛血清样品中葡萄糖的检测。

A method of visible spectral analysis for trace glucose measurement based on the chelating system of Fe^3＋-squaric acid was constructed. Glucose can be oxidized to gluconic acid and H2O2 by the catalysis of glucose oxidase（ GOx）. The produced H2O2 can oxidized Fe^2＋to Fe^3＋. The generated Fe^3＋can produce a significant color change through reaction with squaric acid,so as to establish a method for glucose measurement. Results showed the absorbance of Fe^3＋-squaric acid was linear with the concentration of glucose in the range of 0. 01 - 1. 0 mmol/L（ R = 0. 9962） with a detection limit of 0. 005 mmol/L. Compared with commercially available blood glucose meter,the current method had a higher sensitivity with good stability in 8 h. The proposed method was applied to the determination of glucose in fetal bovine serum samples with satisfactory results and possessed a good application prospect.