十二烷基羟丙基磺基甜菜碱对产膜表皮葡萄球菌粘附作用的影响

Effect of dodecyl hydroxypropyl sulfobetaine on adhesion of Staphylococcus epidermidis biofilm formation bacteria

通过表面活性剂十二烷基羟丙基磺基甜菜碱(DSB)对表皮葡萄球菌生物被膜菌粘附的抑制作用研究,为抗由表皮葡萄球菌生物被膜菌引起的相关感染提供新的探索方向和治疗途径。以万古霉素为阳性对照,利用XTT减低法评价DSB对表皮葡萄球菌初始粘附的影响。发现质量浓度为64 mg/L的DSB对表皮葡萄球菌初始粘附有显著的抑制作用;用质量浓度为512,256和128 mg/L的DSB对实验材料预处理后,对表皮葡萄球菌初始粘附的抑制作用明显。结果表明,DSB对表皮葡萄球菌形成生物被膜的初始粘附过程具有显著的抑制作用。

An investigation of inhibitory effects of surfactant dodecyl hydroxypropyl suffobetaine （DSB） against adhesion of Staphylococcus epidermidis biofilm formation bacteria was conducted in order to find possible new research direction and treatment method of S. epidermidis biofilm bacteria associated infection. Vancomycin is applied as positive control, xTr reduction assay is used to evaluate the effect of DSB on the initial adhesion of S. epidermidis. The presented results show that DSB displays a significantly inhibitory effect on the initial adhesion of S. epidermidis at the mass concentration of 64 mg/L. After the experimental starting materials are pretreated by DSB at the mass concentration of 512,256 and 128 rag/L, the initial adhesion of S. epidermidis is significantly inhibited. Therefore, experimental results indicate that DSB has an significantly inhibitory effects on the initial adhesion in the process of biofilm formation of S. epidermidis.