Musashi2在结直肠癌组织中的表达及其临床意义

Clinicopathological significance of Musashi 2 expression in human colorectal cancer

目的探讨结直肠癌（colorectal cancer，CRC）组织中Musashi2（MSI2）蛋白和mRNA表达水平及其临床意义。方法应用免疫组织化学方法检测85例配对的CRC和癌旁组织中MSI2蛋白的表达水平，再以免疫印迹和实时定量PCR检测12例CRC和配对癌旁组织中MSI2蛋白和mRNA的表达水平。结果85例CRC组织中MSI2表达高于癌旁组织（59％比30％，P〈0．01）。MSI2高表达与CRC患者的肿瘤大小（r=7．682，P=0．006）、T分期（x^2=4．218，P=0．040）、Dukes分期（r=8．590，P=0．014）以及Ki67表达（x^2=6．412，P=0．011）均呈正相关。高表达MSI2的CRC患者预后不良（x^2=4．855，P=0．028）。MSI2在12例CRC组织中蛋白和mRNA的表达高于癌旁组织（t=3．323，P〈0．01；t=2．673，P=0．022）。结论CRC组织中MSI2高表达与患者的肿瘤大小、T分期、Dukes分期、Ki67表达及不良预后密切相关。

Objective To study the clinicopathological significance of the expression of musashi2 （ MSI 2） protein and mRNA levels in human colorectal cancer （CRC）. Methods The expression of MSI 2 protein in 85 CRC specimens and paired adjacent non-cancerous tissues were detected by immunohistochemistry （IHC）. The relationship between the protein expression and clinicopathological features was analyzed. Immunoblotting and real time quantitative PCR were used to examine the expression of MSI 2 protein and mRNA levels in 12 paired fresh CRC and adjuvant non-cancerous tissues. Results MSI 2 overexpression was found in 45 cases of 75 CRC tissues, which was much higher than that in non- cancerous tissues （59% vs. 30% , P 〈 0. 01 ）. MSI 2 overexpression had a positive correlation with tumor size （x^2 =7.682, P=0.006）, T stage （A/ =4.218, P=0.040）, Dukes stage （x^2 =8.590, P=0.014）, and Ki67 expression （x^2 = 6. 412, P = 0. 011 ）. Moreover, C RC patients with MSI 2 overexpression had a worse prognosis （x^2 =4. 855, P =0. 028）. Both MSI 2 protein and mRNA levels in 12 eases of CRC tissues were much higher than that in non-cancerous tissues （ t = 3. 323, P 〈 0. 01 ; t = 2. 673, P = 0. 022, respectively）. Condusion MSI 2 overexpression is closely related with tumor size, T stage, Dukes stage, Ki67 expression and poor prognosis of CRC patients.