甜瓜细菌性果斑病菌鉴定及杀菌剂毒力测定

Identification of Bacterial Fruit Blotch and Toxicity Test of Fungicides

以内蒙古发病甜瓜上分离的病原细菌为试材,采用形态学、生理生化测定和16SrRNA序列比对的方法,进行了病原菌的生物学鉴定,并通过离体试验和活体试验相结合的方法,研究了不同杀菌剂对致病菌的防治效果,以期为细菌性果斑病的防治提供参考依据。结果表明:试验菌株为西瓜食酸菌(Acidovorax citrulli),毒力测定结果显示,不同杀菌剂对西瓜食酸菌的毒力差异较大,同一杀菌剂在离体和活体试验中的结果也不相同,需要2种试验方法相结合,才能对药剂生物活性作出准确评价。

The pathogenic bacteria were isolated and purified from the melon of Inner Mongolia.The morphological,physiological and biochemical tests and 16 SrRNA sequence alignment were used to identify the pathogens.The results showed that the effects of different fungicides on pathogenic bacteria were studied by the combination of in vitro and in vivo experiments,and the virulence was evaluated comprehensively.Identification result showed that the strain was Acidovorax citrulli.The results showed that the virulence of different fungicides to pathogens varied greatly,and the effect of the same fungicides in vitro and in vivo was different.Two methods were needed to evaluate the bioactivity of the fungicides.