喷施沼液对苹果产量品质及蔗糖代谢相关酶活性的影响

Effect of Spraying Biogas Slurry on Apple Yield,Quality and Sucrose Metabolism Enzyme Activity

以短枝"富士"苹果为试材,研究了叶面喷施不同体积分数的沼液对苹果产量品质及成熟过程中蔗糖代谢相关酶活性的影响。结果表明:喷施体积分数为75%的沼液,增加了果实维生素C、可溶性总糖和可滴定酸含量以及果实的果形指数,改善了苹果果实品质,提高了苹果的产量,与对照不喷清水不喷肥相比增产率最高,为13.7%,即667m^2可增产44.5kg。果实成熟后期,各喷肥处理下的蔗糖合成酶和蔗糖磷酸合成酶活性均高于对照CK,随着沼液喷施体积分数的增加,蔗糖含量逐渐增多;蔗糖含量与蔗糖合成酶和蔗糖磷酸合成酶活性呈极显著正相关,相关系数为0.648、0.530(P<0.01)。因此,可以在苹果成熟后期叶面喷施沼液增加蔗糖合成酶和蔗糖磷酸合成酶的活性,进而提高果实内蔗糖含量,改善苹果的品质。

Short shoot ‘Fuji＇apple was taken as the test materials,the effect of different volume fraction of biogas slurry spraying on the leaf on apple production quality and sucrose metabolism related enzyme activity in the course of mature were studied.The results showed that,biogas slurry spraying volume fraction of 75%,increased the fruit vitamin C,soluble sugar and titratable acid content and fruit shape index,improved the apple fruit quality,improved the production of apple,and controlled neither spray water or spray fertilizer production rate was the highest,reached to 13.7%,which could increase 44.5kg per 667m^2.During the later ripe,the spray fertilizer treatment of sucrose synthase and sucrose phosphate synthase were higher than CK,with the biogas slurry spraying volume fraction increasing,sucrose content increased;sucrose content and the activity of sucrose synthase and sucrose phosphate synthase were extremely significant positive correlated,the correlation coefficient was 0.648,0.530（P0.01）,on the basis of that,the synthesis of sucrose in the process of mature of‘Fuji＇apples might be mainly regulated by sucrose synthase and sucrose phosphate synthase.And spraying biogas slurry on the leaf could increase its activity in the late mature,thus improved the sucrose content in apple fruit.