p16^(INK4a)与Notch1基因在宫颈病变组织中的表达及其差异分析

Expression of p16^(INK4a) and Notch1 Gene in Cervical Lesions and Their Difference Analysis

目的:通过检测p16^(INK4a)与Notch1基因在宫颈病变中的表达及其差异分析,筛选出灵敏度和特异性高的宫颈癌早期诊断生物标记物.方法:通过免疫组化SP法对病理学检查后的187例宫颈病变患者的石蜡组织切片进行p16^(INK4a)和Notch1蛋白表达情况检测,其中43例宫颈癌、26例重度宫颈上皮内瘤变(CIN-Ⅲ)、28例中度宫颈上皮内瘤变(CIN-Ⅱ)、30例轻度宫颈上皮内瘤变(CIN-Ⅰ)、34例子宫平滑肌瘤、26例慢性宫颈炎.结果:p16^(INK4a)蛋白在宫颈癌、CIN-III、CIN-II、CIN-I、子宫平滑肌瘤和慢性宫颈炎中阳性表达率分别为100.00%(43/43)、92.31%(24/26)、57.14%(16/28)、53.33%(16/30)、41.18%(14/34)、30.77%(8/26),差异有显著性意义(P<0.05);Notch1蛋白在宫颈癌、CIN-III、CIN-II、CIN-I、子宫平滑肌瘤和慢性宫颈炎中阳性表达率分别为83.72%(36/43)、46.15%(12/26)、57.14%(16/28)、53.33%(16/30)、44.12%(15/34)、61.54%(16/26),差异有显著性意义(P<0.05);在相同类型宫颈病变组织中p16^(INK4a)与Notch1的检测结果阳性表达率有显著性差异(P<0.05).结论:经比较分析p16^(INK4a)与Notch1两基因在宫颈病变中的表达,p16^(INK4a)基因灵敏度和特异性均良好,可以将p16^(INK4a)蛋白作为宫颈癌早期诊断的生物标记物.

Objective： By detecting the expression of p16^（INK4a）and Notch1 gene in cervical lesions and analyzing their expression difference,to screen out the high sensitive and specific biomarkers for early diagnosis of cervical cancer.Methods： The expressed p16^（INK4a）and Notch1 protein was detected by immunohistochemistry S-P method in paraffin sections of 187 patients with cervical lesions after pathological examination,of which 43 cases were cervical cancer,26 cases were severe cervical intraepithelial neoplasia（CIN-Ⅲ）,28 cases were moderate cervical intraepithelial neoplasia（CIN-Ⅱ）,30 cases were mild cervical intraepithelial neoplasia（CIN-Ⅰ）,34 cases were uterine leiomyoma,26 cases were chronic cervicitis. Results： The positive expressionrate of p16^（INK4a）gene in cervical cancer,CIN-Ⅲ,CIN-Ⅱ,CIN-Ⅰ,uterine leiomyoma and chronic cervicitis was 100.00%（43/43）,92.31%（24/26）,57.14%（16/28）,53.33%（16/30）,41.18%（14/34）and 30.77%（8/26）respectively,there was significant difference（P 0.05）. The positive expression rate of Notch1 gene in cervical cancer,CIN-Ⅲ,CIN-Ⅱ,CIN-Ⅰ,uterine leiomyoma and chronic cervicitis was 83.72%（36/43）,46.15%（12/26）,57.14%（16/28）,53.33%（16/30）,44.12%（15/34）and 61.54%（16/26）respectively,there was significant difference（P0.05）. The positive expression rates of p16^（INK4a）and Notch1 in the same type of cervical lesions had significant difference（P 0.05）. Conclusion： By comparing the expression of p16^（INK4a）and Notch1 two genes in cervical lesions,the sensitivity and specificity of p16^（INK4a）gene is better than Notch1,so the p16^（INK4a）protein could be used as a biomarker for early diagnosis of cervical cancer.