鹿角粉复合支架与纳米级羟基磷灰石复合支架的性能比较

Performance comparison of the antler powder composite scaffolds and nano-hydroxyapatite composite scaffolds

目的探索不同胶体材料结合鹿角粉和纳米级羟基磷灰石(n-HA)支架的理化、生物性能。方法取鹿角粉和纳米级羟基磷灰石,分别与15%聚乙烯醇(PVA)、5%丝素蛋白液(SF)、15%PVA和5%丝素蛋白液(4∶1)混合,制备鹿角粉/PVA支架、鹿角粉/SF支架、鹿角粉/PVA/SF支架、n-HA/PVA支架、n-HA/SF支架、n-HA/PVA/SF支架,检测其抗溶解性能、抗压缩力、抗剪切力及支架细胞毒性。结果鹿角粉/SF支架、n-HA/SF支架溶解变形程度为Ⅲ级,其余4组为Ⅱ级。鹿角粉/SF支架、n-HA/SF支架抗剪切力、抗压缩力均较差,鹿角粉/PVA支架、鹿角粉/PVA/SF支架分别与n-HA/PVA支架、nHA/PVA/SF支架抗剪切性能相似,鹿角粉/PVA支架、鹿角粉/PVA/SF支架抗压缩性能分别优于n-HA/PVA支架、n-HA/PVA/SF支架。鹿角粉/PVA支架、鹿角粉/SF支架、鹿角粉/PVA/SF支架细胞相容性优于n-HA/PVA支架、n-HA/SF支架、n HA/PVA/SF支架。结论鹿角粉复合支架具有良好的机械性能及细胞相容性,可以作为异种骨支架材料研究的新方向。单纯丝素作为水凝胶复合支架抗溶解性不佳,机械性能差,无法满足骨组织工程支架要求。

Objective To explore the physicochemical and biological properties of different colloidal materials combined with antler powder and nano-hydroxyapatite （n-HA） scaffolds.Methods Antler powder and nano-hydroxyapatite meal were mixed with 15% PVA,5% silk fibroin （SF）,15% PVA and 5% silk fibroin （4 ∶ 1） respectively to prepare antler powder/PVA scaffolds,antler powder/SF scaffolds,antler powder/PVA/SF scaffolds,nano-hydroxyapatite （n-HA）/PVA scaffolds,nano-hydroxyapatite （n-HA）/SF scaffolds,nano-hydroxyapatite （n-HA）/PVA/SF scaffolds.The anti-dissolution performance,anti-compression force,shear resistance and scaffold cytotoxicity were measured.Results The dissolution deformation of antler powder/SF scaffolds and n-HA/SF scaffolds were grade Ⅲ,and the other four groups were grade Ⅱ.The shear resistance and anti-compression force of the antler powder/SF scaffolds and n-HA/SF scaffolds were poor.The anti-shear properties of the antler powder/PVA scaffolds and antler powder/PVA/SF scaffolds were similar to those of the n-HA/PVA scaffolds and n-HA/PVA/SF scaffolds.The anti-compression properties of antler powder/PVA scaffolds and antler powder/PVA/SF scaffolds were better than n-HA/PVA scaffolds and n-HA/PVA/SF scaffolds,respectively.Conclusions Antler powder composite scaffolds have good mechanical properties and cell compatibility,and can be used as a new direction for heterogeneous bone scaffold materials.Pure silk fibroin,as a composite scaffold with poor solubility,poor mechanical properties,cannot meet the requirements of bone tissue engineering scaffolds.