摘要
目的探索不同胶体材料结合鹿角粉和纳米级羟基磷灰石(n-HA)支架的理化、生物性能。方法取鹿角粉和纳米级羟基磷灰石,分别与15%聚乙烯醇(PVA)、5%丝素蛋白液(SF)、15%PVA和5%丝素蛋白液(4∶1)混合,制备鹿角粉/PVA支架、鹿角粉/SF支架、鹿角粉/PVA/SF支架、n-HA/PVA支架、n-HA/SF支架、n-HA/PVA/SF支架,检测其抗溶解性能、抗压缩力、抗剪切力及支架细胞毒性。结果鹿角粉/SF支架、n-HA/SF支架溶解变形程度为Ⅲ级,其余4组为Ⅱ级。鹿角粉/SF支架、n-HA/SF支架抗剪切力、抗压缩力均较差,鹿角粉/PVA支架、鹿角粉/PVA/SF支架分别与n-HA/PVA支架、nHA/PVA/SF支架抗剪切性能相似,鹿角粉/PVA支架、鹿角粉/PVA/SF支架抗压缩性能分别优于n-HA/PVA支架、n-HA/PVA/SF支架。鹿角粉/PVA支架、鹿角粉/SF支架、鹿角粉/PVA/SF支架细胞相容性优于n-HA/PVA支架、n-HA/SF支架、n HA/PVA/SF支架。结论鹿角粉复合支架具有良好的机械性能及细胞相容性,可以作为异种骨支架材料研究的新方向。单纯丝素作为水凝胶复合支架抗溶解性不佳,机械性能差,无法满足骨组织工程支架要求。
Objective To explore the physicochemical and biological properties of different colloidal materials combined with antler powder and nano-hydroxyapatite (n-HA) scaffolds.Methods Antler powder and nano-hydroxyapatite meal were mixed with 15% PVA,5% silk fibroin (SF),15% PVA and 5% silk fibroin (4 ∶ 1) respectively to prepare antler powder/PVA scaffolds,antler powder/SF scaffolds,antler powder/PVA/SF scaffolds,nano-hydroxyapatite (n-HA)/PVA scaffolds,nano-hydroxyapatite (n-HA)/SF scaffolds,nano-hydroxyapatite (n-HA)/PVA/SF scaffolds.The anti-dissolution performance,anti-compression force,shear resistance and scaffold cytotoxicity were measured.Results The dissolution deformation of antler powder/SF scaffolds and n-HA/SF scaffolds were grade Ⅲ,and the other four groups were grade Ⅱ.The shear resistance and anti-compression force of the antler powder/SF scaffolds and n-HA/SF scaffolds were poor.The anti-shear properties of the antler powder/PVA scaffolds and antler powder/PVA/SF scaffolds were similar to those of the n-HA/PVA scaffolds and n-HA/PVA/SF scaffolds.The anti-compression properties of antler powder/PVA scaffolds and antler powder/PVA/SF scaffolds were better than n-HA/PVA scaffolds and n-HA/PVA/SF scaffolds,respectively.Conclusions Antler powder composite scaffolds have good mechanical properties and cell compatibility,and can be used as a new direction for heterogeneous bone scaffold materials.Pure silk fibroin,as a composite scaffold with poor solubility,poor mechanical properties,cannot meet the requirements of bone tissue engineering scaffolds.
作者
赵小琦
周琦琪
韩祥祯
宋艳艳
向晓松
何惠宇
ZHAO Xiaoqi ZHOU Qiqi HAN Xiangzhen SONG Yanyan XIANG Xiaosong HE Huiyu(Department of Prosthodontics, the First Affili- ated Hospital of Xinjiang Medical University, Urumqi 830054, Chin)
出处
《口腔医学》
CAS
2017年第9期791-795,共5页
Stomatology
基金
国家自然科学基金项目(81660177)
关键词
鹿角粉
纳米级羟基磷灰石
支架材料
聚乙烯醇
丝素蛋白
antler powder
nano-hydroxyapatite
scaffold material
polyvinyl alcohol
silk fibroin solution