TREK-1介导大鼠牙移动颅面疼痛的机制研究

The role of TREK-1 in orofacial pain induced by experimental tooth movement in rats

目的建立大鼠牙移动所致的颅面疼痛模型,阐明离子通道TREK-1在介导颅面疼痛中的作用。方法 80只6周龄雄性SD大鼠,随机分成4组:单纯加力组、对照组、氟西汀(Fluoxetine)治疗组和奎尼丁(Quinidine)治疗组。根据X线片测量牙移动的距离;使用RGS大鼠疼痛级别评价指数进行疼痛评价;使用免疫组化染色观察牙周组织中TREK-1的表达。结果实验组大鼠第一磨牙近中移动量随加力时间增加而增大,且在14 d达到最大量的前移;加力组大鼠疼痛评分在各时间点均高于对照组,且疼痛随着加力时间的增加先增大后减小,在加力3 d时,疼痛最明显;加力3 d时,第一磨牙根间牙周组织血管壁高表达TREK-1;TREK-1的化学性抑制剂氟西汀(Fluoxetine)和奎尼丁(Quinidine)均能降低牙移动引起的疼痛,且不影响牙移动的速度和距离。结论 TREK-1介导大鼠牙移动过程中颅面疼痛的产生,通过抑制TREK-1可以在不影响牙移动效率的基础上缓解牙移动引起的颌面部疼痛。

Objective To establish a craniofacial pain model induced by tooth movement in rats,and to elucidate the role of potassium channel TREK-1 in mediating craniofacial pain.Methods Eighty female Sprague-Dawley rats were randomly divided into four groups： the experimental group,the control group,Fluoxetine treated group and Quinidine treated group.The distance of the tooth movement was measured by the X-ray films; the degree of pain was evaluated using the RGS index,and the expression of TREK-1 in the periodontal tissue was observed by immunohistochemical staining.Results The mesial movement of the first molar in the experimental group increased over time and reached the maximum amount on 14 th day.The pain score of the rats in the experiment group was higher than that of control group and the most obvious pain occurred on 3rd day; on 3rd day,TREK-1 highly expressed in the periodontal vascular wall; Fluoxetine and Quinidine （chemical inhibitors of TREK-1） reduced the pain caused by tooth movement,and did not affect the speed and distance of tooth movement.Conclusion TREK-1 mediates the development of craniofacial pain during tooth movement in rats.TREK-1 can relieve the maxillofacial pain caused by tooth movement without affecting the efficiency of tooth movement.