花果山云雾茶中多酚和多糖的超声同步提取工艺

Ultrasonic-assisted Synchronous Extraction Process of Tea Polyphenols and Polysaccharides from Huaguoshan Yunwu Tea

研究花果山云雾茶中茶多酚和茶多糖的超声辅助同步提取工艺并评价提取物的抗氧化活性。在单因素试验的基础上,响应面分析法优化的提取工艺为:超声功率500 W,提取温度80℃,提取时间37 min,液料比62∶1(mL/g)。在优化的条件下,茶多酚和茶多糖的得率分别为(137.08±3.37)mg/g和(57.71±1.94)mg/g。固态提取物的得率为(2.51±0.27)%,其中茶多酚和茶多糖的含量分别为(548.31±13.48)mg/g和(227.28±6.44)mg/g。通过测定提取物的还原力、抗氧化力和对DPPH自由基、羟自由基及超氧阴离子自由基的清除能力评价其抗氧化活性,结果显示提取物具有较强的还原力和总抗氧化力及清除DPPH自由基、羟自由基和超氧阴离子自由基的活性。

The ultrasonic-assisted synchronous extraction conditions of tea polyphenols（TPP）and tea polysaccharides（TPS）from Huaguoshan Yunwu tea and antioxidant activities of the extracts were investigated. On the basis of single factor experiments,Box-Behnken design and response surface methodology were applied to optimize the extraction conditions. The optimal extraction conditions were as follows：ultrasonic power 500 W,extraction temperature 80 ℃,extraction time 37 min,liquid to material ratio 62∶1（mL/g）. According to the optimal process conditions,the yield of TPP and TPS were（137.08±3.37）mg/g and（57.71±1.94）mg/g dry basis.The yield of solid ultrasonic-assisted extracts（UAE）was（2.51±0.27）%,the contents of TPP and TPS were（548.31±13.48）mg/g and（227.28±6.44）mg/g UAE respectively. The antioxidative activities of UAE were estimated by measuring reducing power and total antioxidant capacity,the scavenging activities on DPPH free radical,hydroxyl free radical and superoxide anion free radical. The results indicated that UAE had the strong reducing power and total antioxidant capability,as well as the high eliminating activities on DPPH free radical,hydroxyl free radical and superoxide anion free radical.