探究激活态雪旺细胞与生物可降解聚己内酯支架的生物相容性

Study on the biocompatibility of PCL scaffold with activated Schwann cells

目的:探究激活态雪旺细胞(ASC)、非激活态雪旺细胞(SC)与生物可降解聚己内酯(PCL)支架的生物相容性。方法:实验组由结扎7 d Wistar大鼠坐骨神经提取激活态雪旺细胞,对照组由未结扎大鼠坐骨神经提取非激活态雪旺细胞。将细胞分别种植在PCL支架上,通过CCK-8检测细胞增殖活性,通过结晶紫观察细胞分布,激光共聚焦显微镜观察细胞形态和功能表达。结果:ASC与SC相比S-100阳性率无明显差异;PCL支架上ASC增殖率明显高于SC;ASC能够贴附PCL纤维生长,可沿纤维长轴分布,进入支架内部,并且表达髓鞘碱性蛋白(MBP)。结论:激活态雪旺细胞比非激活态雪旺细胞能够更好地在PCL支架上生长,PCL支架具有良好的生物相容性。

Objective： To study the biocompatibility of polycaprolactone（PCL） scaffold with activated Schwann cells（ASC） and normal Sehwann cells（SC）. Methods：There were two groups： ASC extracted from ligated sciatic nerves experiment group; SC extracted from normal sciatic nerves control group. Proliferation activity was tested through the CCK- 8 test, and the distribution and shape of cells was observed by crystal violet staining and confoeal laser scanning microscope. Results： There was no difference between ASC and SC in the positive rate of S-100; the proliferation rate of ASC was significantly higher than that of SC in PCL scaffold; ASC could attach PCL fiber and move along the long axis of the fiber into the scaffolds, and express myelin basic protein （MBP）. Conclusion：Activated Schwann cells can grow better in PCL scaffold than non-activated Schwann cells, and PCL scaffold has good hiocompatibility.