内皮祖细胞对背根神经节细胞突起及Nogo-A和NgR表达的影响

Effects of endothelial progenitor cells on dorsal root ganglion neurites and the expression of Nogo-A and NgR

目的:观察大鼠内皮祖细胞(EPCs)对乳鼠背根神经节(DRG)细胞突起影响,通过Nogo-A和NgR表达变化探究其可能机制。方法:培养DRG和制备EPCs后分别鉴定,实验组DRG和EPCs共培养,对照组2份等量的DRG共培养。第2和4日分别计算两组DRG突起数量、突起最长和平均长度,统计两组DRG细胞纯度和活性,检测Nogo-A和NgR及mRNA表达。结果:实验组DRG细胞突起数量、突起最长和平均长度在共培养第2和4日较对照组均明显增长(P<0.05),实验组DRG细胞纯度和活性均高于对照组(P<0.05),实验组Nogo-A和NgR mRNA表达量在共培养第2和4日均显著降低(P<0.05),Western blot结果示实验组Nogo-A和NgR蛋白表达量在第2和4日明显低于对照组(P<0.05)。结论:EPCs通过介导Nogo-A和NgR表达下调,促进DRG细胞突起生长。

Objective： To explore the effect of endothelial progenitor cells （EPCs） on dorsal root ganglions （DRG） neurites by the expression of Nogo-A and NgR. Methods： DRG and the EPCs were prepared and identified separately. EPCs co-culturing with DRG was designed as the experimental group, and DRG co-culturing with DRG was designed as the control group. The number of neurites, the maximum length of neurites, the average length, the purity and vitality were calculated by Image J software on day 2 and day 4. Correspondingly Nogo-A, NgR mRNA and protein were detected. Results： The number of DRG neurites, the maximum length of DRG neurites and the average length elevated dramatically （P〈0.05）. The purity and vitality of DRG in experimental group were significantly higher （P〈0.05）. Nogo-A and NgR mRNA in experimental group were significantly lower on day 2 and day 4 （P〈0.05）. Corresponding protein expression of Nogo-A and NgR in experimental group were significantly lower （P〈0.05）. Conclusion： EPCs could restraint the expression of Nogo-A and NgR, which may markedly promote the growth of DRG neurites.