AIF和HSP70在胃息肉及胃癌中的表达及其相关性研究

Expressions of apoptosis inducing factor and Heat-shock protein 70 in gastric polyps and gastric cancer and their correlation with gastric cancer

目的检测凋亡诱导因子(AIF)及热休克蛋白70(HSP70)mRNA在胃癌及胃息肉中的表达,探讨其对胃癌的发生、发展的影响。方法收集内蒙古医科大学第三附属医院120例活检标本,依据病理分为胃增生性息肉组、胃腺瘤性息肉组、胃癌组、正常胃黏膜组,每组30例。采用实时荧光定量PCR检测相应胃黏膜组织中AIF mRNA与HSP70 mRNA的表达水平。结果在胃增生性息肉、胃腺瘤性息肉及胃癌组织中,AIF及HSP70 mRNA的表达呈逐渐上升趋势,差异有统计学意义(P<0.05);在胃腺瘤性息肉组及胃癌组中,AIF mRNA与HSP70 mRNA的表达呈正相关(P<0.01)。结论 AIF及HSP70基因可能与胃癌形成有关,均可作为胃癌早期诊断的临床指标,AIF mRNA和HSP70 mRNA可能在胃癌的形成及发展过程中发挥协同作用,可能为基因的协同治疗提供新靶点。

Objective To explore the effects of AIF and HSP70 in the occurrence and development of gastric cancer by detecting their mRNA levels in gastric polyps and gastric cancer, and provide a new way and insight for the early di- agnosis and treatment of gastric cancer. Methods Gastric biopsy samples of 120 patients in the Third Hospital of Inner Mongolia Medical University were collected. According to their pathological characteristics, the samples were divided in- to four groups as followed ： normal gastric mucosa group （ n = 30） , gastric hyperplastic polyps group （ n = 30） , pol- ypoidabenoma group （n = 30） and gastric cancer group （n = 30）. The expressions of AIF and HSP70 mRNA were detected by real-time PCR. Results The expressions of AIF and HSP70 mRNA were gradually increased in gastric byperplastic polyps, gastril adenomatous polyp and gastric cancer, the difference was significant （P 〈 0.05）. In the tissues of adenomatous polyp and gastric cancer, the expression of AIF mRNA was positively correlated with HSP70 mRNA （P 〈 0.01 ）. Conclusion AIF and HSP70 are correlated with the development of gastric cancer. AIF and HSP70 may act synergistically in the development of gastric cancer. Both of them can be used as markers for the diagnosis of gastric cancer.