5种杀虫剂对3个种群马铃薯桃蚜ATPase的抑制作用

Inhibition of Five Insecticides on ATPase Activity of Myzus persicae on Potato

[目的]探寻呼和浩特和武川地区马铃薯桃蚜对杀虫剂的不敏感性与其体内ATP酶的关系。[方法]应用ATP酶测定试剂盒,分别测定了呼和浩特、武川种群马铃薯桃蚜和敏感种群桃蚜神经系统Na^+-K^+-ATP酶和Ca^(2+)-Mg^(2+)-ATP酶的活性及5种杀虫剂对各种群体内这2种ATP酶活性的抑制作用。[结果 ]3个种群桃蚜的Na^+-K^+-ATP酶和Ca^(2+)-Mg^(2+)-ATP酶活性存在显著性差异,敏感种群桃蚜的ATPase活性最高,武川种群的ATPase活性最低。5种杀虫剂在抑制终浓度为1×10^(-4) mol/L时对3个种群桃蚜离体ATP酶活性均有一定程度的抑制作用,其中氰戊菊酯对3个种群桃蚜离体ATPase活性抑制最强,并且对敏感种群的抑制作用高于呼市和武川种群桃蚜。[结论]呼和浩特和武川地区马铃薯桃蚜体内2种ATP酶对药剂的敏感性已明显下降。这2种ATP酶可能参与了呼和浩特和武川种群抗药性的形成。

[Aims] This study aims to explore the relationship between insensitivity of peach aphid Myzua persicae （Sulzer） to insecticides and their ATPase in Hohhot and Wuchuan area. [Methods] The activity of Na＋-K＋-ATPase and Ca^2＋-Mg^2＋-ATPase of were determined with ATPase kit in Hohhot, Wuchuan and sensitive population. The inhibition of five pesticides on Na＋-K＋-ATPase and Ca2＋-Mg^2-ATPase in M. persicae were also tested. [Results] The results showed that there was significant difference in Na＋-K＋-ATPase and Ca2＋-Mg^2＋-ATPase activities among three strains. ATPase activity of sensitive population was the highest and that of Wuchuan was the lowest. Five insecticides inhibited obviously the two mentioned ATPase activities in three populations. The inhibition of fanvalerate on two ATPase was the strongest, and the inhibition of fanvalerae on ATPase in M. persicae in sensitive population was higher than that of other two populations. [Conclusions] The sensitivities of two ATPase ofM. persicae to pesticides decreased in Hob_hot and Wuchuan area. Na＋-K＋-ATPase and Ca^2＋-Mg^2＋-ATPase might even play some part in the form of resistant to insecticides of M. persicae in Hohhot and Wuchuan area.