老年期痴呆典型证候的演变机制研究

Study on the Evolution Mechanism of Typical Syndromes of Senile Dementia

目的通过构建老年期痴呆肾虚精亏证和痰浊阻窍证动物模型,观察大鼠睾酮(T)、白介素6(IL-6)、谷胱甘肽过氧化物酶(GSH-Px)含量的变化,探讨老年期痴呆典型证候演变规律。方法先分次永久结扎双侧颈总动脉,然后腹腔注射D-半乳糖建立老年期痴呆大鼠模型,在疾病造模的同时,分别采用过度房劳体虚和投喂高脂饲料的方法,建立病证结合肾虚精亏证和痰浊阻窍证的大鼠模型。造模结束后对各组大鼠进行行为学检测,并测定其血清内T、IL-6和GSH-Px的含量。结果与正常组比较,老年期痴呆组、肾虚精亏证组、痰浊阻窍证组大鼠的学习记忆能力以及空间搜索能力均下降(P<0.05);与老年期痴呆组相比,肾虚精亏证组大鼠的T和GSH-Px含量明显降低(P<0.05),痰浊阻窍证组大鼠的IL-6含量显著升高(P<0.05)。结论老年期痴呆典型证候演变以肾虚为主线,贯穿于痴呆发病始终,表现出因虚致实、虚实夹杂的特点。

Objective By establishing animal models of senile dementia with syndromes of kidney vacuity and sperm depletion and phlegm obstructing orifices,we observed the changes in the volume of big mice＇s testosterone（ T）,Interleukin-6（ IL-6） and Glutathione peroxidase（ GSH-Px）,and studied the evolving rules of senile dementia＇s typical syndromes. Methods Firstly,permanent ligation on bilateral common carotid artery separately was operated. Then mice models with senile dementia by injecting D-galactose into their stomachs were established. When making disease models,we used over-sexual strain and feeding high-fat diet separately to establish models with syndromes of kidney vacuity and sperm depletion and models with syndromes of phlegm obstructing orifices. After that,we did behavioristic test on the mice of each group and examined the volume of T,IL-6 and GSH-Px in serum. Results Comparing with the control group,the learning and memory ability and space searching ability of senile dementia group,kidney vacuity and sperm depletion group and phlegm obstructing orifices group all decreased（ P〈0. 05）. Comparing with senile dementia group,T and GSH-Px＇s volume of mice in kidney vacuity and sperm depletion group also decreased obviously（ P〈0. 05）,and the volume of IL-6 of phlegm obstructing orifices group increased obviously（ P〈0. 05）. Conclusion Kidney vacuity is the main syndrome of senile dementia patients,which appears at the beginning of the disease and keeps with it. It is featured by ＂excess caused by deficiency＂and ＂intermingled deficiency and excess＂.