火针对脊髓损伤大鼠神经干细胞Wnt/?-catenin通路基因表达的离体研究

In Vitro Study on Fire Needling and the Expression of Wnt/b-catenin Pathway Genes in Neural Stem Cells in Rats with Spinal Cord Injury

目的观察火针对脊髓损伤Wnt/?-catenin通路上神经干细胞Wnt、?-catenin基因的调控作用。方法将60只SD雌性大鼠随机分为空白组5只、假手术组5只、模型组25只、火针组25只,模型组和火针组大鼠又分为1 d组、3 d组、7 d组、10 d组和14 d组;采用改良的Allen's法对模型组和火针组造模,观察各组BBB评分,Wnt3a和?-catenin基因表达变化。结果在7 d、10 d时模型组和火针组BBB评分明显高于同组前一时相,差异具有统计学意义(P<0.05);火针组大鼠在治疗后各时间点BBB评分明显高于模型组,差异具有统计学意义(P<0.05)。在7 d、10 d时模型组和火针组Wnt3a基因表达量较同组前一时相均增加(P<0.05);在7 d、10 d时火针组Wnt3a基因表达量高于模型组,差异具有统计学意义(P<0.05);?-catenin基因表达水平变化趋势与Wnt3a基本一致。结论火针可调控脊髓损伤神经干细胞Wnt3a、?-catenin基因表达。

Objective To investigate the regulating effect of fire needling on Wnt and β-catenin genes of Wnt/β-catenin pathway in neural stem cells in spinal cord injury. Methods Sixty female SD rats were randomly allocated, including 5 rats to a blank group, 5 rats to a sham operation group, 25 rats to a model group and 25 rats to a fire needling group. The model and fire needling groups of rats were again separately divided into 1 d, 3 d, 7 d, 10 d and 14 d groups. A model was made using modified Allen＇s method in the model and fire needling groups. The BBB score was recorded and the expressions of Wnt and β-catenin genes were determined in every group. Results In the model and fire needling groups, the BBB scores were significantly higher at 7 and 10 days than at the previous time point and there was a statistically significant difference （P〈0.05）. The BBB score at every time point after treatment was significantly higher in the fire needling group than in the model group and there was a statistically significant difference （P〈0.05）. Wnt3a gene expressions increased in the model and fire needling groups at 7 and 10 days compare with the previous time point （P〈0.05）. At 7 and 10 days, Wnt3a gene expression was higher in the fire needling group than in the model group and there was a statistically significant difference （P〈0.05）. The changing tendency of β-catenin gene expression levels was basically the same as that of Wnt3a＇s. Conclusion Fire needling can modulate the expressions of Wnt3a and β-catenin genes in neural stem cells in spinal cord injury.