微波辅助提取虫草花中多糖成分的研究

Study on the Microwave Assist Extraction of the Polysaccharides from the Caterpillar Fungus

以食材辅料虫草花为研究对象,采用微波辅助法研究提取虫草花中多糖成分,利用红外光谱法检验脱脂后石油醚的团变化情况,用苯酚-硫酸法测定虫草花多糖。实验表明微波提取功率为140 W,辐射4 min,紫外分光光度计测定多糖含量,比色测定溶液在487 nm波长处有最大吸收,相关线性关系y=13.8032x+0.2233,R^2=0.9932,适用于蛹虫草子实体中多糖含量测定。

Use the caterpillar fungus flowers saccharine was extracted by microwave assisted method technology research. Then, the use of infrared spectroscopy testing after decreasing group change situation of petroleum ether, determine the petroleum ether to caterpillar fungus flowers decreasing effect of non polar solution. Wave method of forceps saccharine extracted, using phenol-sulfuric acid method for determination of forceps saccharine. The result shows that wave power is 140 W, the time is 4 min, and at the wave-light of 487 nm, the linear equation is Y=13.8032X＋0.2233, R2=0.9932. The method is use for caterpillar fungus flowers and competent definite.