粉单竹竹青色素提取工艺及其成分分析

Extraction Technology and Composition Analysis of Bamboo Bark Pigment from Bambusa Chungii

以乙醇为提取溶剂,采用超声波提取粉单竹竹青色素,通过单因素实验,获得竹青色素最佳提取条件为:乙醇浓度90%,料液比1∶35,超声时间40 min,超声温度55℃,提取两次。实验结果表明,在最佳提取条件下粉单竹竹青叶绿素提取率为0.39%;色素纯化后通过柱层析分离得到橙黄色、蓝绿色、黄色、绿色4种色素,在紫外可见光区(400~800 nm)测4种色素紫外光谱图,发现该图形分别与叶绿素a、叶绿素b和两种类胡萝卜素的谱图相似;通过核磁共振分析,纯化后的提取物中可能含有脂肪酸,甾醇等;通过红外光谱分析可知,提取液中含有羟基、C=C双键、C=O双键、C—O键等官能团。

Bamboo bark pigment was extracted from BambusaChungii by ultrasonic extraction, using ethanol as extraction solvent. The optimum extraction conditions were as the follows ： ethanol concentration 90%, solidliquid ratio 1 ： 35, ultrasonic time 40 min, ultrasonic temperature 55℃. After twice extraction under the optimum condition, the pigment was basically extracted completely, the extraction rate of chlorophyll was about O. 39%. Pigment was purified and separation by column chromatography to obtain orange pigment, turquoise pigment, yellow pigment and green pigment, then graphics of Uv-vis spectrophotometer were similar to chlorophyll a, chlorophyll b and two carotenoids, and the purified extracting may also contain fatty acids, sterols and so on identific by nuclear magnetic spectrum（ NMR）, the purified extracting contained -OH, C =C, C =O, C-O and so on by fourier transform infrared spectroscopy（FT-IR） analysis.