似鲇高原鳅皮肤与鳍的组织学观察

Histology of Skin and Fins in Catfish-like Loach (Triplophysa siluroides)

采用石蜡切片技术及H.E和AB-PAS染色方法,对似鲇高原鳅(Triplophysa siluroides)头部、腹部、背部、侧线部和尾部皮肤结构及胸鳍、腹鳍、背鳍、臀鳍和尾鳍的组织结构进行观察。各部位皮肤均由表皮和真皮构成,真皮包括疏松层和致密层,不同部位皮肤厚度不同。表皮层腹部最厚,为(84.62±10.82)μm,侧线部最薄,为(14.97±3.95)μm,各部位表皮厚度差异显著。表皮层分布着黏液细胞、棒状细胞及味蕾。疏松层头部最厚,为(282.71±70.56)μm,尾部最薄,为(29.07±4.88)μm,该层分布有黑色素细胞、空泡状细胞和颗粒腺,而黏液腺分布于致密层。各部位的鳍均由表皮层、胶原纤维层、胶原下层及鳍条构成,表皮层与皮肤表皮层组成相似,鳍条是矿化的结缔组织。

The structural characteristics of the skin and fins were observed by paraffin section, haematoxylin-eosin and Alcian blue-periodic acid Schiff staining in Catfish-like Loach （Triplophysa siluroides）. Skin samples were taken from head, abdomen, dorsal, lateral and caudal peduncle, respectively, and fins samples were taken from pectoral fin, ventral fin, dorsal fin, anal fin and caudal fin. The comparison of difference between groups was analyzed with one-way ANNVOA method using SPSS 17.0. The results showed that the skins were all composed of epidermis and dermis, and the latter was divided into stratum spongiosum and stratum compactum （Plate I ）. Different parts of skins had different thicknesses which was related to the size of the glands （Table 1, Table 2）. Among the five different positions, the thickest epidermis layer （84.62 ± 10.82 μm） was present in the abdomen, while the thinnest epidermis （14.97 ± 3.95 μm） was present in lateral region, and there was significant difference in epidermis thickness at different positions （Table 1）. Mucous cells, club cells and taste buds were observed in the epidermis layer. For stratum spongiosum, the thickest layer （282.71 ±70.56 μm） was in the head, while the thinnest layer （29.07± 4.88 μm） was in the caudal peduncle （Table 1）. There were some pigment cells, vacuolar cells and granular glands in the stratum spongiosum, and the mucous glands were present in the stratum compactum （Plate I ）. The thickness of stratum spongiosum could be thicker due to the presence of glands. Fins were composed of epidermis layer, collagen fiber layer, sub-collagen fiber layer and fin ray. The composition of epidermis layer was similar with that in the skin. Fin ray was mineralized connective tissue （Plate I ）.