阿魏酸钠对同型半胱氨酸致人脐静脉内皮细胞功能障碍的影响及机制

Action mechanism of sodium ferulate on dysfunction of human umbilical vein endothelial cells induced by homocysteine

目的:采用人脐静脉内皮细胞建立了同型半胱氨酸血管内皮细胞损伤模型,研究阿魏酸钠对胞外同型半胱氨酸损伤的内皮细胞功能障碍的保护作用。方法:采用CCK8法检测不同浓度阿魏酸钠对人脐静脉内皮细胞活性的影响;人脐静脉内皮细胞与不同浓度阿魏酸钠和同型半胱氨酸共同孵育后,直接观察同型半胱氨酸对人脐静脉内皮细胞形态的影响,检测一氧化氮、一氧化氮合成酶、内皮素、丙二醛、超氧化物歧化酶、谷胱甘肽过氧化物酶含量变化及相应阿魏酸钠的保护作用。结果:阿魏酸钠能够减少同型半胱氨酸对人脐静脉内皮细胞的损伤,降低同型半胱氨酸对人脐静脉内皮细胞产生的氧化压力,减轻同型半胱氨酸诱导的人脐静脉内皮细胞分泌功能异常。结论:阿魏酸钠能够缓解同型半胱氨酸导致的人脐静脉内皮细胞功能障碍。

OBJECTIVE A model of extracellular homocysteine induced injury in human vascular endothelial cell（HUVEC）was established to evaluate the protective role of sodium ferulate on dysfunction of homocysteine induced endothelial cells injury.METHODS The viability of HUVEC was determined by CCK8 assay.After coincubation with or without different concentrations of sodium ferulate and homocysteine,the morphology of HUVEC was determined,superoxide dismutase,malondialdehyde,glutathione peroxidase,nitric oxide,nitric oxide synthase and endothelin-1 were measured in culture medium.RESULTSSodium ferulate can reduce the homocysteine induced change of endothlial morphology.Further studies also showed that sodium ferulate might reduce the homocysteine induced oxidative stress and partly modulate the secretary dysfunction of vascular endothelial cells induced by homocysteine.CONCLUSION Sodium ferulate may be beneficial in prevention of endothelial dysfunction caused by homocysteine.