摘要
目的:测定维药青香茅中芹菜素-7-O-β-D-吡喃葡萄糖苷含量。方法:采用高效液相色谱法,使用SinoChrom C18色谱柱(5μm,250 mm×4.6 mm),以甲醇-0.2%磷酸水溶液(41∶59)为流动相,检测波长324 nm。结果:芹菜素-7-O-β-D-吡喃葡萄糖苷在5.25~52.50μg·mL^(-1)范围内与峰面积呈良好的线性关系(r=0.999 9),方法平均回收率为:97.29%(RSD为1.23%),平均含量为57.912 1μg·g^(-1)。结论:本方法简便,准确,重复性好,可用测定维药青香茅中芹菜素-7-O-β-D-吡喃葡萄糖苷含量。
OBJECTIVE To determine apigenin-7-O-β-D-glucopyranoside from the Uygur Cymbopogon caesius(Ness)Stapf.METHODS Determination was performed by high performance liquid chromatography(HPLC)with C18column(5μm,250mm×4.6 mm),with mobile phase of methanol-0.2% phosphoric acid solution(41∶59),detection wavelength at 324 nm.RESULTS Apigenin-7-O-β-D-glucopyranoside concentration had a good linear relationship with peak area in range of 5.25–52.50g·mL^(-1)(r=0.999 9).The average recovery rate was 97.29%(RSD=1.23%).The average content of apigenin-7-O-β-D-glucopyranoside in Cymbopogon caesius(Ness)Stapf.was 57.912 1μg·g-1.CONCLUSION This method is simple,accurate and reliable,and can be used for the quality control of Uygur Cymbopogon caesius(Ness)Stapf.
作者
杨颖
王莹
刘婧怡
郝宇薇
耿直
兰卫
YANG Ying WANG Ying LIU Jing-yi HAO Yu-wei GENG Zhi LAN Wei(Xinjiang Huashidan Phar- maceutical Co.. Ltd. , Xinjiang Urumuqi 830011, China Affiliated TCM Hospital of Xinjiang Medical University, Xinjiang Urumqi 830002, China College of Traditional Chinese Medicine, Xinjiang Medical University, Xinjiang Urumuqi 830011, China)
出处
《中国医院药学杂志》
CAS
北大核心
2017年第17期1713-1715,共3页
Chinese Journal of Hospital Pharmacy
基金
新疆维吾尔自治区科技援疆项目(201491173)