摘要
目的探讨CCL5/CCR5生物轴在涎腺腺样囊性癌(SACC)细胞嗜神经侵袭中的作用。方法应用细胞免疫荧光技术和流式细胞术检测SACC-LM细胞中趋化因子受体CCR5的表达;应用酶联免疫吸附测定(ELISA)法检测周围神经细胞培养上清液中趋化因子CCL5的表达。应用流式细胞术检测CCL5对SACC-LM细胞内F肌动蛋白的作用,并通过划痕和侵袭实验观察趋化因子CCL5和其受体CCR5对SACC-LM细胞运动能力和侵袭能力的作用。结果 SACC-LM细胞高表达趋化因子受体CCR5;周围神经原代培养上清液中趋化因子CCL5质量浓度为(359.2±15.8)、(696.4±22.6) pg·m L-1;趋化因子CCL5和其受体CCR5结合后对SACC-LM细胞的运动能力和侵袭能力可以产生促进作用。结论 CCL5/CCR5生物轴可能在SACC细胞嗜神经侵袭中起着重要的作用。
Objective This study aimed the role of the CCL5/CCR5 axis in the perineural invasion of salivary adenoid cystic carcinoma （SACC） cells. Methods Immunohistochemical analysis and flow cytometric analysis were conducted to detect the expression of the chemokine receptor CCR5 in SACC cells. Enzyme linked immunosorbent assay （ELISA） was performed to determine the expression of CCL5 in the supernate of human nerve cells. The flow cytometric analysis was applied to observe the changes in F-actin in SACC-LM cells, which were pretreated with CCL5. To assess the effects of the CCL5/CCR5 axis on the migration and invasion of SACC-LM cells, we performed a scratch test and invasion assay under CCL5 stimulation. Results CCR5 was highly expressed in SACC cells. The concentration of CCL5 in the supernatant of human nerve cells was （359.2±15.8）, （696.4±22.6） pg·mL-1. The CCL5/CCR5 axis promoted the migration and invasion of SACC-LM cells. Conclusion The CCL5/CCR5 axis may be involved in the perineural invasion of SACC cells.
作者
李添翼
申志远
Li Tianyi Shen Zhiyuan(Dept. of Pediatric Dentistry, Stomatological Hospital of Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China Dept. of Oral and Maxillofacial Surgery, Stomatological Hospital of Shandong University, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250012, China)
出处
《华西口腔医学杂志》
CAS
CSCD
北大核心
2017年第5期479-483,共5页
West China Journal of Stomatology
