水酶法提取葡萄籽油和蛋白质的研究

Aqueous enzymatic extraction process of oil and protein from grape seeds

为充分利用葡萄酿酒后的副产物,对水酶法提取葡萄籽中的油脂和蛋白质的工艺进行优化,并研究不同干燥方法和提取方法对葡萄籽油提取量和理化性质的影响。结果表明:最优提取条件为纤维素酶、果胶酶、木聚糖酶、胰蛋白酶质量比2∶1∶2∶1、p H 6.5、料液比1∶9、酶解时间9 h,在此条件下,葡萄籽油提取量为99.47 mg/g,蛋白质提取量为74.86 mg/g,蛋白质提取率为57.58%;不同干燥方法对水酶法提取葡萄籽油的提取量和理化性质影响显著,从油的提取量、品质和成本考虑,在生产中应优先选择风干的干燥方式;与传统方法相比,水酶法提取的葡萄籽油品质更好,并可以直接得到副产物蛋白质,但是提取效率不够理想,有待进一步研究。

In order to make full use of byproducts of grapes after brewing, the extraction processes of oil and protein from grape seeds by aqueous enzymatic method were optimized, and the effects of different dr- ying and extraction ways on the extraction amount and physicochemical properties of srape seed oil were investigated. The results showed that the optimal extraction conditions were obtained as follows： mass ra- tio of cellulose, pectinase, xylanase and trypsin 2：1 ： 2： 1, pH 6.5, ratio of material to liquid 1 ： 9 and enzymolysis time 9 h. Under these conditions, the extraction amount of grape seed oil, extraction amount and extraction rate of protein were 99.47, 74.86 mg/g and 57.58% had obvious influences on the extraction amount and physicochemical sideration from extraction amount of oil, quality Compared with traditional methods, the quality was better, and the protein byproducts could be not ideal enough, which need further studies. and cost, air drying respectively. Different drying ways properties of grape seed oil. Con- should be preferred in production. of grape seed oil extracted by aqueous enzymatic method obtained directly. However, the extraction efficiency was